Status:
Advanced ReviewerMember since: 2019
Organization: Keck of USC
Great product.
Application Area: Protein extract from cultured cells
"The procedure is very easy and fast. Results are reliable and reproducible."
Subcellular Protein Fractionation Kit includes a combination of reagents for stepwise lysis of cells into functional cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal protein fractions in less than 3 hours. Extracts from each subcellular compartment generally have less than 15% contamination between fractions, which is sufficient purity for most experiments studying protein localization and redistribution.
The Subcellular Protein Fractionation Kit for Cultured Cells contains four extraction buffers, a stabilized nuclease and Thermo Scientific Halt Protease Inhibitor Cocktail. Each kit has enough reagents to fractionate 50 cell pellets of 20 µL each, equivalent to approximately 2g of cell paste.
Features:
Manufacturer Thermo Fisher Scientific
4.7 / 5.0 | 1 reviews