Thermo Scientific™ Subcellular Protein Fractionation Kit for Cultured Cells

Subcellular Protein Fractionation Kit includes a combination of reagents for stepwise lysis of cells into functional cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal protein fractions in less than 3 hours. Extracts from each subcellular compartment generally have less than 15% contamination between fractions, which is sufficient purity for most experiments studying protein localization and redistribution.

The Subcellular Protein Fractionation Kit for Cultured Cells contains four extraction buffers, a stabilized nuclease and Thermo Scientific Halt Protease Inhibitor Cocktail. Each kit has enough reagents to fractionate 50 cell pellets of 20 µL each, equivalent to approximately 2g of cell paste.

Features:

• Concise—extract functional cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal protein fractions from mammalian cultured cells in less than 3 hours
• Convenient—perform a simple procedure without using ultracentrifugation over gradients
• Cultured Cells—developed and optimized for use with cultured mammalian cells
• Compatible—use extracts for downstream applications such as protein assays, Western blotting, protein assays, electrophoretic mobility shift assays, reporter-gene and enzyme-activity assays.