Thermo Scientific™ Q Exactive™ HF Hybrid Quadrupole-Orbitrap™ Mass Spectrometer
Identify and quantify more proteins, peptides, lipids, glycans and small molecules accurately and in less time with the Thermo Scientific™ Q Exactive™ HF hybrid quadrupole-Orbitrap mass spectrometer.
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Precision and accurate results.
Human doping urine analysis
Best system for screening analysis of urine samples of athletes in human doping. Orbitrap mass spectrometers offer the highest levels of accuracy and precision. Benefit from the high-resolution, accurate-mass (HRAM) performance advantages offered by Thermo unique Orbitrap mass analyzers. Orbitrap systems connect to liquid chromatography equipment to enhance separation of unknown and known compounds and enable high-throughput workflows.
Review Date: 23 Jul 2021 | Thermo Fisher Scientific
Q Exactive mass spectrometers rock!
Analysis of blood and urine samples for forensic and clinical applications & retrospective analysis
I have been using the Q Exactive-HF coupled to a Thermo Vanquish LC system for about 2.5 years and have developed two screens (one in urine and one in blood). The urine drug screen is used to analyze samples from hospital emergency departments whereas the blood drug screen is used for the analysis of post-mortem blood samples. I also look at new psychoactive substances retrospectively to figure at what new drugs should be added to the screens for monitoring. This is the function of the instrument that has always excited me the most, being able to go back to acquired data and retrospectively and interrogate it further. I have found many new NPS using this technique. The QE-HF has been extremely stable for the past 2.5 years. Both LC and MS require very little maintenance. Calibration is done once a week and the mass accuracy hardly shifts, despite our lab temperature fluctuating quite a bit. Other than the PM, we hardly see the Thermo engineers. The instrument is used continuously (I would say that we are at 90% capacity right now) and gives us little trouble. The application scientists at Thermo were wonderful when we started and continue to help us every time we have a question. Although I am in Canada, I had specialists from Germany helping out on a particular problem. Their whole team is truly available for you. The reason why I didn't give them 5 stars is that it takes time to get the instrument fixed if it goes down. There is no warehouse in Vancouver or Canada. Everything comes from the States and if there is a snowstorm or another weather event, the delivery of parts takes a long time. If it comes from Europe it is longer. I wish Thermo had a small warehouse in Canada. As for the value for money, I truly think we have it. The instrument delivers on all accounts for us: sensitivity, reproducibility, robustness, ease of use (Tracefinder is a very well-designed software). I will always value a robust instrument that doesn't break down. I have used Q Exactive Plus in the past, coupled with a Dionex 3000 and had the same wonderful experience. Highly reliable instruments. All in all, my favourite instrument in the lab.
Review Date: 7 Jul 2021 | Thermo Fisher Scientific
Highly recommended.
Identification of low MW unknowns
The software is intuitive and robust. The hardware can take significant abuse before charging effects become noticeable. Tuning is simple and quick. One of Thermo's best Orbitrap instruments.
Review Date: 9 Apr 2021 | Thermo Fisher Scientific
The Swiss army knife of mass spectrometers!
Metabolomics and lipidomics
I cannot rave about this mass spectrometer enough: It's easy to use; robust; reliable; and, having trained two technicians who had zero experience in mass spectrometry on it, I can say it could not be more intuitive or user friendly. I have used it for all sorts of applications looking at small molecules: Metabolomics, Lipidomics, PKPD, DNA/RNA modifications. It has never failed to deliver. Money well spent indeed! The only reason for not giving the after-sales care a five-star review is because their field service engineers are too few and their escalation processes too rigid, which causes repairs - especially complex ones - to last longer than they should. Otherwise, I have nothing but good things to say about Thermo Fisher Scientific products and assistance.
Review Date: 19 Feb 2021 | Thermo Fisher Scientific
Great reliable and useful instrument.
Global studies
It's a very reliable and sturdy product. I am very happy with the service too.
Review Date: 11 Jul 2019 | Thermo Fisher Scientific
Great instrument with easy handling and high quality results.
Proteomics and metabolomics
Orbitrap from Thermo Scientific has a user-friendly design. They developed a fairly un-complicated software to run the machine. It has so far produced very reliable data and we were able to compare our data with different labs. I highly recommend this machine to new users for proteomics and metabolomics. Capturing of high-quality MS data and reflecting also low abundant analytes is an important strength of the instrument.
Review Date: 15 Mar 2019 | Thermo Fisher Scientific
Reliable instrument, not much can go wrong.
Proteomics
Instrument easy to use, good results, instrument calibration is straight forward. Il highly recommend this instrument for day to day running.
Review Date: 15 Mar 2019 | Thermo Fisher Scientific
Complete, fast, high reproducibility
Metabolomics
User friendly. In metabolomic applications it enables the detection and the identification of new compounds.
Review Date: 16 Apr 2018 | Thermo Fisher Scientific
Great Instrument
Drug EFS proteomics chemistry
I am getting high quality results. Will recommend to others.
Review Date: 16 Apr 2018 | Thermo Fisher Scientific
Advanced quadrupole technology for greater sensitivity and quantitative dynamic range
The Q Exactive HF instrument uses a segmented quadrupole mass filter to achieve more precise precursor isolation for better discrimination between analytes and coeluting interferences. An advanced active beam guide increases ion transmission. The result is superior sensitivity and greater quantitative dynamic range, especially when dealing with complex mixtures.
Multiple, sophisticated modes of data acquisition/quantitation ensure optimum quantitative results
The Q Exactive HF mass spectrometer supports multiple approaches to quantitation including selected ion monitoring (SIM), parallel-reaction monitoring (PRM), and data-independent acquisition (DIA). Users can select the technique best matched to their sample and information requirements.
- SIM - selected ion monitoring provides simple method setup with maximum selectivity and sensitivity. It is ideal for quantifying small numbers of compound in samples of moderate complexity.
- PRM - parallel-reaction monitoring provides high selectivity and high sensitivity with confident targeted confirmation. Quantitation is carried out post-acquisition for greater throughput. High-resolution, accurate-mass Orbitrap spectra ensure confident confirmation through library matching.
- DIA - data independent acquisition enables large-scale targeted proteomics studies with qualitative confirmation. Its simple and universal acquisition set-up does not require detailed sample knowledge prior to data acquisition. DIA increases reproducibility and comprehensiveness with a complete record of quantitative data. It facilitates retrospective analysis of additional analytes.
Ultra-high-field Orbitrap mass analyzer increases resolving power and scan speed for greater productivity and higher confidence in less time
The Q Exactive HF system features a new ultra-high-field Orbitrap mass analyzer that, while physically smaller, provides higher resolution and faster scan speeds. Combined with the inherent superior quality of Orbitrap spectra, the Q Exactive HF system provides more-confident identification and quantitation in less time
- Scan speed up to 18 Hz result in up to 50% more IDs from shorter gradients
- Resolving power of up to 240,000 (FWHM) at m/z 200 ensures maximum ID confidence
- Spectral quality significantly superior to Q-TOF mass spectrometers yields better protein coverage and quantitation results
- Polarity switching (one full cycle in <1 sec at a resolution setting of 60,000) enables analysis of more, different compounds in a single analysis
Optional intact protein mode for enhanced analysis of intact proteins and protein complexes
When research requires analysis of intact proteins or protein complexes, the Q Exactive HF system can be equipped with an intact protein mode option (available on new instruments only). This option enhances analysis of intact proteins through sophisticated ion beam control and easy pressure adjustment.
