Member since: 2015
Organization: Johns Hopkins University
Great silver stain reagent.
Application Area: Protein gel staining
"I've been using this product for about 4-5 years now. Safe, easy to use, quick, and reproducible."
The Pierce Silver Stain Kit is a metallic silver protein stain that yields a remarkably clear and uniform gel background while delivering consistent, high-sensitivity staining results at each and every use. In standard mini gels, proteins are detectable at greater than 0.25ng per band or spot. The protocol has been optimized for flexibility by allowing short or overnight gel fixation and staining steps without affecting staining performance (sensitivity or clarity). A short (one-minute) sensitization step, performed after gel fixation, yields results that are free of the characteristically dark or blotchy backgrounds often seen with homemade or other commercially available silver stains. This feature is beneficial when performing densitometric analysis of silver stained gels.