Thermo Scientific™ Pierce™ Silver Stain Kit by Thermo Fisher Scientific

Manufacturer Thermo Fisher Scientific  |   Model: 24612
4.3
/
5.0
  |  1 reviews
The Pierce Silver Stain Kit is a rapid, ultra-sensitive and versatile silver stain system for protein detection in polyacrylamide gels, yielding consistent and reliable results with many 1D and 2D gel types


Thermo Scientific™ Pierce™ Silver Stain Kit by Thermo Fisher Scientific product image
Thermo Scientific™ Pierce™ Silver Stain Kit

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Average Rating: 4.3
1 Scientist has reviewed this product

5 out of 5
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2015

  • Organization: Johns Hopkins University



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Great silver stain reagent.
Rating: 4.3

  • Application Area: Protein gel staining

"I've been using this product for about 4-5 years now. Safe, easy to use, quick, and reproducible."

Review date: 30 Nov 2018 | Thermo Scientific™ Pierce™ Silver Stain Kit

The Pierce Silver Stain Kit is a metallic silver protein stain that yields a remarkably clear and uniform gel background while delivering consistent, high-sensitivity staining results at each and every use. In standard mini gels, proteins are detectable at greater than 0.25ng per band or spot. The protocol has been optimized for flexibility by allowing short or overnight gel fixation and staining steps without affecting staining performance (sensitivity or clarity). A short (one-minute) sensitization step, performed after gel fixation, yields results that are free of the characteristically dark or blotchy backgrounds often seen with homemade or other commercially available silver stains. This feature is beneficial when performing densitometric analysis of silver stained gels.


Features:

  • Sensitive—detect proteins at 0.25 ng; equal or better sensitivity than monochromatic metallic silver stains from other suppliers
  • Clear background—method produces intensely-stained protein gels with very low background color, shading or anomalies
  • Robust performance—reagents and protocol are formulated to yield excellent staining results amidst even wide-ranging differences in user technique
  • Rapid, flexible protocol—approx. 1.5 hours are required to complete the default procedure
  • Gel-compatible—staining performance is very good with a variety of commercial precast mini-gels and SDS-PAGE buffer-systems
  • Workflow-compatible—mild chemical formulation ensures compatibility with mass spectrometry, sequencing and other downstream procedures that depend on destaining and protein recovery