Member since: 2020
So convenient, such a time saving kit!
Application Area: PCR - genotyping
"This product is very easy to use and allows us to cut our genotyping time by several times (few minutes for the lysis instead of an hour). The quality is optimal: we can use tiny amounts of DNA (like small parts of E7.5 embryos) for genotyping and it still works great."
Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects. The Master Mix containing Phire Hot Start II DNA Polymerase is specially formulated to perform PCR in the presence of different animal tissue-derived inhibitors such as collagen, melanin and eumelanin (hair, skin) or myoglobin (muscle). The kit also includes Thermo Scientific DNARelease Additive, which can be used to improve the release of DNA from difficult tissues.