Member since: 2020
Organization: Institute for Biomedic Research of A Coruna (INIBIC)
Straight forward, good results.
Application Area: Protein fractionation
"This kit is quite fast and easy to use, but nuclear pellet lysis efficiency depends heavily on the kind of cells used (some require more incubation time and syringe lysis before the final centrifugation step). Nuclear and cytoplasmic fractions are quite pure according to Lamin A-tubulin levels."
Member since: 2019
Organization: Westminster College
Very easy to use and good results and yeild of DNA
Application Area:Used to extract plasmids for use in DNA transformation
"The prep kit was very easy to use and fairly inexpensive. The students were able to get good results and yield of DNA and help the experiment move much faster"
Member since: 2012
Organization: University Texas Southwestern Med Ctr
Application Area:Nuclear and Cytosolic protein isolation from Drosophila tissues and cell lines
"This extraction kit works really well. The step by step instructions are really easy to follow and perform. We get very good yield and purity of nuclear and cytoplasmic fractions."
The Thermo Scientific NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours.
The NE-PER Kit is a nuclear protein extraction method that involves simple, stepwise lysis of cells and centrifugal isolation of nuclear and cytoplasmic protein fractions. A benchtop microcentrifuge, tubes and pipettors are the only tools required. The NE-PER Reagents efficiently solubilize and separate cytoplasmic and nuclear proteins into fractions with minimal cross-contamination or interference from genomic DNA and mRNA. Once desalted or diluted, the isolated proteins can be used to perform immunoassays and protein interaction experiments, such as mobility shift assays (EMSA), co-immunoprecipitation (Co-IP) and pull-down assays.