Thermo Scientific NE-PER Nuclear and Cytoplasmic Extraction Kit by Thermo Fisher Scientific
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Status:
Reviewer
Member since: 2021
Organization: Southern Illinois University School of Medicine
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- Ease of use
- After sales service
- Value for money
Great result.
Application Area: Molecular biology
"It's an easy to use kit. Applicable to research in gram positive and negative bacteria."
Review date: 13 Oct 2021 | Thermo Scientific NE-PER Nuclear and Cytoplasmic Extraction Kit
Status:
Expert Reviewer Member since: 2020
Organization: Institute for Biomedic Research of A Coruna (INIBIC)
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- Ease of use
- After sales service
- Value for money
Straight forward, good results.
Application Area:Protein fractionation
"This kit is quite fast and easy to use, but nuclear pellet lysis efficiency depends heavily on the kind of cells used (some require more incubation time and syringe lysis before the final centrifugation step). Nuclear and cytoplasmic fractions are quite pure according to Lamin A-tubulin levels."
Review date: 12 Dec 2020 | Thermo Scientific NE-PER Nuclear and Cytoplasmic Extraction Kit
Status:
Reviewer Member since: 2019
Organization: Westminster College
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- Ease of use
- After sales service
- Value for money
Very easy to use and good results and yeild of DNA
Application Area:Used to extract plasmids for use in DNA transformation
"The prep kit was very easy to use and fairly inexpensive. The students were able to get good results and yield of DNA and help the experiment move much faster"
Review date: 03 Jun 2019 | Thermo Scientific NE-PER Nuclear and Cytoplasmic Extraction Kit
Status:
Reviewer Member since: 2012
Organization: University Texas Southwestern Med Ctr
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- Ease of use
- After sales service
- Value for money
Application Area:Nuclear and Cytosolic protein isolation from Drosophila tissues and cell lines
"This extraction kit works really well. The step by step instructions are really easy to follow and perform. We get very good yield and purity of nuclear and cytoplasmic fractions."
Review date: 22 Dec 2014 | Thermo Scientific NE-PER Nuclear and Cytoplasmic Extraction Kit
The Thermo Scientific NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours.
The NE-PER Kit is a nuclear protein extraction method that involves simple, stepwise lysis of cells and centrifugal isolation of nuclear and cytoplasmic protein fractions. A benchtop microcentrifuge, tubes and pipettors are the only tools required. The NE-PER Reagents efficiently solubilize and separate cytoplasmic and nuclear proteins into fractions with minimal cross-contamination or interference from genomic DNA and mRNA. Once desalted or diluted, the isolated proteins can be used to perform immunoassays and protein interaction experiments, such as mobility shift assays (EMSA), co-immunoprecipitation (Co-IP) and pull-down assays.
Features:
- Fast – obtain nuclear and cytoplasmic fractions of soluble proteins in less than two hours
- Proven – the NE-PER Reagent Kit is referenced in more than 950 distinct publications
- Versatile – nuclear protein from either cultured cells or tissues (intended for fresh samples only)
- Scalable – two kit sizes for producing extracts from cells and tissues
- Convenient – simple instructions do not require ultracentrifugation over gradients
- Compatible – use for downstream assays, including Western blotting, gel-shift assays, protein assays, reporter gene assays and enzyme activity assays
Product Overview
Thermo Scientific NE-PER Nuclear and Cytoplasmic Extraction Kit
Manufacturer Thermo Fisher Scientific
4.5 / 5.0 | 4 reviews
