Thermo Scientific NE-PER Nuclear and Cytoplasmic Extraction Kit by Thermo Fisher Scientific

4.9
/
5.0
  |  2 reviews
Prepare concentrated nuclear extracts for an EMSA and gel shift assays. The Thermo Scientific NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours. The NE-PER Kit is a nuclear protein extraction method that involves simple, stepwise lysis of cells and centrifugal isolation of nuclear and cytoplasmic protein fractions. A benchtop microcentrifuge, tubes and pipettors are the only... Read more


Thermo Scientific NE-PER Nuclear and Cytoplasmic Extraction Kit by Thermo Fisher Scientific product image
Thermo Scientific NE-PER Nuclear and Cytoplasmic Extraction Kit

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Average Rating: 4.9
2 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2019

  • Organization: Westminster College



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Very easy to use and good results and yeild of DNA
Rating: 5.0

  • Application Area: Used to extract plasmids for use in DNA transformation

"The prep kit was very easy to use and fairly inexpensive. The students were able to get good results and yield of DNA and help the experiment move much faster"

Review date: 03 Jun 2019 | Thermo Scientific NE-PER Nuclear and Cytoplasmic Extraction Kit
  • Status:

    Reviewer

  • Member since: 2012

  • Organization: University Texas Southwestern Med Ctr



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Rating: 4.7

  • Application Area:Nuclear and Cytosolic protein isolation from Drosophila tissues and cell lines

"This extraction kit works really well. The step by step instructions are really easy to follow and perform. We get very good yield and purity of nuclear and cytoplasmic fractions."

Review date: 22 Dec 2014 | Thermo Scientific NE-PER Nuclear and Cytoplasmic Extraction Kit
Prepare concentrated nuclear extracts for an EMSA and gel shift assays.

The Thermo Scientific NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours.

The NE-PER Kit is a nuclear protein extraction method that involves simple, stepwise lysis of cells and centrifugal isolation of nuclear and cytoplasmic protein fractions. A benchtop microcentrifuge, tubes and pipettors are the only tools required. The NE-PER Reagents efficiently solubilize and separate cytoplasmic and nuclear proteins into fractions with minimal cross-contamination or interference from genomic DNA and mRNA. Once desalted or diluted, the isolated proteins can be used to perform immunoassays and protein interaction experiments, such as mobility shift assays (EMSA), co-immunoprecipitation (Co-IP) and pull-down assays.

Features:

  • Fast – obtain nuclear and cytoplasmic fractions of soluble proteins in less than two hours
  • Proven – the NE-PER Reagent Kit is referenced in more than 950 distinct publications
  • Versatile – nuclear protein from either cultured cells or tissues (intended for fresh samples only)
  • Scalable – two kit sizes for producing extracts from cells and tissues
  • Convenient – simple instructions do not require ultracentrifugation over gradients
  • Compatible – use for downstream assays, including Western blotting, gel-shift assays, protein assays, reporter gene assays and enzyme activity assays

Product Overview

Thermo Scientific NE-PER Nuclear and Cytoplasmic Extraction Kit by Thermo Fisher Scientific product image

Thermo Scientific NE-PER Nuclear and Cytoplasmic Extraction Kit

Manufacturer Thermo Fisher Scientific

4.9 / 5.0 | 2 reviews