Thermo Scientific™ EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride) by Thermo Fisher Scientific

Manufacturer Thermo Fisher Scientific  |   Model: 77149
4.3
/
5.0
  |  1 reviews
Pierce EDC is a water-soluble carbodiimide crosslinker that activates carboxyl groups for spontaneous reaction with primary amines, enabling peptide immobilization and hapten-carrier protein conjugation.


Thermo Scientific™ EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride) by Thermo Fisher Scientific product image
Thermo Scientific™ EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride)

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Average Rating: 4.3
1 Scientist has reviewed this product

5 out of 5
Ease of use
3 out of 5
After sales service
5 out of 5
Value for money


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  • Organization: Jamia Millia Islamia New Delhi



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    5 out of 5
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No doubt their solvent is highly recommended for organic chemistry
Rating: 4.3

  • Application Area: Organic synthesis

"Our lab uses the the chemicals which is exceptionally pure. In organic chemistry, specifically medicinal chemistry, purity is a must."

Review date: 25 Jun 2021 | Thermo Scientific™ EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride)

1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC or EDAC) is a zero-length crosslinking agent used to couple carboxyl groups to primary amines. This crosslinker has been used in diverse applications such as forming amide bonds in peptide synthesis, attaching haptens to carrier proteins to form immunogens, labeling nucleic acids through 5' phosphate groups and creating amine-reactive NHS-esters of biomolecules. EDC reacts with a carboxyl to form an amine-reactive O-acylisourea intermediate. If this intermediate does not encounter an amine, it will hydrolyze and regenerate the carboxyl group. In the presence of N-hydroxysulfosuccinimide (Sulfo-NHS), EDC can be used to convert carboxyl groups to amine-reactive Sulfo-NHS esters. This is accomplished by mixing the EDC with a carboxyl containing molecule and adding Sulfo-NHS.


Features:

  • Several conjugation strategies—react EDC alone with target groups or include NHS or Sulfo-NHS to increase reaction efficiency or to stabilize active intermediate for later reaction to amines
  • Neutral linkage—forms neutral amide bonds between carboxyls and amines
  • Water-soluble reagent—add directly to reactions in aqueous, physiological buffers
  • Soluble reaction byproducts—easily removed by washing with water or dilute acid

Product Overview

Thermo Scientific™ EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride) by Thermo Fisher Scientific product image

Thermo Scientific™ EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride)

Manufacturer Thermo Fisher Scientific

4.3 / 5.0 | 1 reviews