Thermo Scientific™ HisPur™ Ni-NTA Resin by Thermo Fisher Scientific

Manufacturer Thermo Fisher Scientific  |   Model: 88222
HisPur Ni-NTA Resin is a high-capacity, high-performance nickel-IMAC resin for routine affinity purification of His-tagged fusion proteins.


Thermo Scientific™  HisPur™ Ni-NTA Resin by Thermo Fisher Scientific product image
Thermo Scientific™ HisPur™ Ni-NTA Resin
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HisPur Ni-NTA Resin effectively purifies high levels of overexpressed His-tagged fusion proteins from bacterial lysates, such as those that are attained with Thermo Scientific B-PER Bacterial Protein Extraction Reagents. The resin performs well in batch-binding and spin-column procedures at a variety of scales. Performance equals or exceeds popular Ni-NTA resins from other suppliers. HisPur Ni-NTA Resin is a high-quality, stable and resilient affinity support. Tests confirm that no decrease in performance occurs after at least five repeated uses. These data indicated that the resin is highly resistant to structural degradation or nickel ion leaching during normal use.


Features:

  • High capacity—bind up to 60 mg of 6xHis-tagged protein per milliliter of resin
  • Versatile—purify proteins using native or denaturing conditions
  • Compatible—use with Thermo Scientific Cell Lysis Reagents and a variety of buffer additives
  • Cost-effective—reuse the same batch of resin at least five times
  • Flexible—available in multiple formats including bulk resin, spin columns, chromatography cartridges and 96-well filter plates