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4 Scientists have reviewed this product
5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
"ELISA kit is easy to use and reproducible. Works well with mouse serum samples. Price for the kit is very reasonable."
Review date: 02 Nov 2018 |
Testosterone ELISA Rating: 5.0
"This product is high quality, easy to use, and arrives fairly quickly. The ordering process is very easy and the pricing is appropriate. I have been using sex steroid hormone ELISAs from DRG for almost six years now and am very satisfied with our results. "
Review date: 30 Jan 2015 |
Testosterone ELISA Rating: 5.0
"The DRG Testosterone kit 1559 is easy to use has consistent high quality and performance, and the packaging is compact. We love this product!"
Review date: 14 Oct 2014 |
Testosterone ELISA Rating: 5.0
"The DRG Testosterone kit 1559 is easy to use, has consistent high quality and performance, and the packaging is compact. We love this product!"
Review date: 01 Sep 2014 |
Testosterone ELISA An enzyme immunoassay for the quantitative measurement of testosterone in serum and plasma. Testosterone (17Beta-hydroxy-4-androstene-3-one) is a C19 steroid with an unsaturated bond between C-4 and C-5, a ketone group in C-3 and a hydroxyl group in the Beta position at C-17. This steroid hormone has a molecular weight of 288.47. Testosterone is the most important androgen secreted into the blood. In males, testosterone is secreted primarily by the Leydig cells of the testes; in females ca. 50% of circulating testosterone is derived from peripheral conversion ofandrostenedione, ca. 25% from the ovary and ca. 25% from the adrenal glands. Testosterone is responsible for the development of secondary male sex characteristics and its measurements are helpful in evaluating the hypogonadal states. In women, high levels of testosterone are generally found in hirsutism andvirilization, polycystic ovaries, ovarian tumors, adrenal tumors and adrenal hyperplasia. In men, high levels of testosterone are associated to the hypothalamic pituitary unit diseases, testicular tumors, congenital adrenal hyperplasia and prostate cancer. Low levels of testosterone can be found in patients with the following diseases: Hypopituitarism, Klinefelter's syndrome, Testicular feminization, Orchidectomy and Cryptorchidism, enzymatic defects and some autoimmune diseases.The DRG Testosterone ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding. The microtiter wells are coated with a monoclonal [mouse] antibody directed towards an unique antigenic site on the Testosterone molecule. Endogenous Testosterone of a sample competes with a Testosterone horseradish peroxidase conjugate for binding to the coated antibody. After incubation the unbound conjugate is washed off. The amount of bound peroxidase conjugate is reverse proportional to the concentration of Testosterone in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Testosterone in the sample.