Member since: 2017
Organization: Driftwood Brewery
Great master mix. Highly suggested.
Application Area: PCR for diagnostics
"This PCR master mix gives me reproducible results, is robust, easy to use, saves time from having to make a new batch of master mix for every PCR run, and is conveniently 2x concentrated."
Applied Biosystems TaqMan® Universal Master Mix II brings sensitivity and precision across a broad range of input target quantities, reliable detection of low copy number targets, and enables accurate quantification to discriminate subtle differences in target abundance.
Stable at room temperature for 24 hours in a preassembled PCR reaction
Validated with Applied Biosystems TaqMan® Assays for gene expression, SNP genotyping, and microRNA research
Uses universal thermal cycling conditions for TaqMan Assays
Can be directly substituted into your existing protocols
Sensitivity and Wide Dynamic Range for Gene Expression Analysis
TaqMan Universal Master Mix II provides dependable target quantification over a wide dynamic range of expression levels, so that you can detect and quantitate high and low expressors in a single experiment.
Specificity to Distinguish Highly Homologous Targets
The master mix also provides excellent specificity in real-time PCR. MicroRNAs are small (21–23 nt) nucleic acids that are involved in the regulation of many genes and have been implicated in disease processes such as cancer and heart disease. Some members of microRNA families differ from each other by as little as 1 nucleotide, presenting a difficult challenge for many master mixes. TaqMan Universal Master Mix II is formulated to deliver the necessary specificity to distinguish between these highly homologous targets.
Benchtop Stability for High-Throughput Handling
TaqMan Universal Master Mix II is formulated with high-quality components that retain the ability to discriminate between small relative differences in target concentration even after incubating assembled PCR reactions for 24 hours at room temperature. This extended benchtop stability provides flexibility to process numerous samples using high-throughput liquid handling systems that may require assembled reactions to sit at room temperature until they can be fed into the real-time PCR instrument.
Validated for TaqMan Genotyping Assays
TaqMan Universal Master Mix II has been validated for use with genotyping assays. The formulation provides well-separated clusters for all categories of assays under universal cycling conditions where samples could be easily called as homozygous or heterozygous.