Member since: 2009
Organization: UNIVERSITY OF OKLAHOMA HSC
Best kit for RNA isolation
Application Area: RNA isolation
"For RNA isolation, this will be the best kit as it gives the purified RNA. This kit is free from phenols and other lysis methods, which makes this kit at first stand. As this kit uses column based method, amount of contamination is very less and gives a good yield of RNA."
Member since: 2016
Organization: NATIONAL POLITECHNIQUE INSTITUTE
Good results, not to much time needed
Application Area:Gene expression of plants
"The SV total RNA Isolation system is an easy and fast way to obtain good quality RNA, the previous preparation of some of the reagents is not complicated. It takes around thirty minutes to complete the protocol, but for someone with plenty experience could achive it in less time. The integrity of the RNA is enough to use in PCR protocols and in the synthesis of cDNA. The protocol include some suggestions that helps to the user."
Isolate RNA with a Simple, 1-hour Procedure
The SV Total RNA Isolation System provides a fast and simple technique for preparation of intact total RNA from tissues, cultured cells and white blood cells in as little as one hour. Using this membrane-based purification system, up to 60mg of tissue can be processed per purification, depending on tissue type. The system incorporates a DNase treatment step directly on the minicolumn membrane. This step substantially reduces genomic DNA contamination, which can interfere with amplification-based methodologies. Purification is achieved without the use of phenol:chloroform extractions or ethanol precipitations, and there is no DNase carryover in the final RNA preparation.