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SureSelect XT HS2 DNA Reagent Kit

Agilent TechnologiesAvailable: Worldwide

The SureSelect XT HS2 DNA Reagent Kit is Agilent’s most cutting-edge library prep and target enrichment solution for Illumina sequencing

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Average Rating 4.8

|2Scientists have reviewed this product

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Great ease of use and automation great robustness

 

Average Rating 4.7

Application Area:

Genome sequencing

Highly sensitive and enable detection of single-nucleotide, DNA/RNA structural and copy number variants, fusions, mutations and insertions and deletions

Review Date: 1 Dec 2023 | Agilent Technologies

Good product for strand-specific RNA library enrichment, for low input samples.

 

Average Rating 5.0

Application Area:

RNA Seq library preparation

Good product for strand-specific RNA library enrichment. Worked well for low input samples.

Review Date: 18 Nov 2021 | Agilent Technologies

The SureSelect XT HS2 DNA Reagent Kit is Agilent’s most cutting-edge library prep and target enrichment solution for Illumina sequencing. It provides a streamlined workflow, excellent performance and comprehensive features that can satisfy most of your NGS library prep needs. The modular configuration enables an easy and parallel workflow for RNA and DNA sequencing using SureSelect XT HS2 chemistry.

This high sensitivity kit is equipped with molecular barcodes to detect low frequency alleles. A total of 384 unique dual indexing allows you to multiplex hundreds of samples and not worry about index hopping. Its ultimate flexibility enables you to choose the workflow that best fits your needs.

Features:

  • Generate high-quality libraries with as little as 10 ng of DNA input from intact or highly fragmented FFPE DNA
  • Satisfy most of your NGS sample prep needs with one streamlined and flexible solution
  • Use single/duplex molecular barcodes (MBC) to remove false positives and accurately detect variants down to ≤1% variant allele frequency
  • Minimize index hopping with 384 unique dual indexing
  • Simplify your order experience with the all-inclusive starter kit
  • Use with SureSelect exomes, catalog panels or easily create custom panels with the SureDesign software
  • One easy, parallel workflow for targeted RNA and DNA sequencing using SureSelect XT HS2 chemistry
Application NoteLife Sciences

Next-generation sequencing FFPE solution

In this application note, Agilent outlines the reagents and software designed to help overcome the challenges of analyzing FFPE samples and support the entire NGS workflow, from quality control and sample preparation to data analysis and clinical interpretation.


How automation and integrated sample QC enhance a targeted long-read-sequencing workflow

Tuesday, November 4, at 16:00 GMT | 17:00 CET | 11:00 EST | 8:00 PST

As the demand for greater accuracy and efficiency in genetic analysis grows, new approaches are needed to overcome the blind spots of conventional sequencing methods. This webinar will showcase a fully automatable workflow that integrates Agilent SureSelect hybrid capture, Agilent automated electrophoresis systems for sample quality control with PacBio HiFi long-read sequencing.

In addition to discussing workflow, we’ll share case studies where long reads play a fundamental role in identifying genetic variants that may be missed by conventional sequencing in research studies.

Join us to learn how targeted long-read sequencing:

  • Focuses on genes and regions of interest in genetic research, while reducing costs and boosting sequencing depth at key loci.
  • Overcomes the limitations of short-read technologies, including challenges with pseudogenes, homologous loci, structural variants, complex transcript isoforms, splicing events, and difficult regions such as repetitive or GC-rich sequences.
  • Enhances both analytical performance and laboratory efficiency through automation.

Key learning objectives:

  • Gain practical insights into implementing an automated targeted long-read sequencing workflow in the laboratory.
  • Identify the genetic mutation types best suited for targeted long-read sequencing approaches.
  • Understand the analytical advantages of long-read sequencing compared with conventional short-read methods.

Who should attend?

Lab directors, molecular pathologists, research scientists, clinical lab technicians, and anyone running or interested in running NGS workflows.

Certificate of attendance
If you attend the live webinar, you will automatically receive a certificate of attendance, including a learning outcomes summary, for continuing education purposes.

If you view the on-demand webinar, you can request a certificate of attendance by emailing editor@selectscience.net.

For Research Use Only. Not for use in diagnostic procedures. PR7003-294


A comprehensive and scalable automation workflow

Agilent Technologies offers a range of instrumentation to automate your specific workflow needs. Automation has always been a strategic cornerstone for Agilent, but even more now than ever before.

As a trusted industry leader, Agilent is committed to delivering innovative and reliable automated technologies. A distinctive strength of the Agilent team and portfolio lies in the extensive breadth of experience and offerings. The Agilent automation platforms address sample preparation needs for proteins, genomics, cell analysis, and metabolomics workflows.

In this video, Agilent presents the Magnis NGS Prep System and Bravo NGS Platforms, designed to help you generate dependable and high-quality NGS data. These scalable and versatile solutions streamline library preparation and target enrichment, to increase sample capacity without compromising on data quality. By embracing automation, laboratories can improve the end-to-end workflow with integrated hardware and protocols tailored to serve a range of application needs.

This interview was filmed at SLAS2024.

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