The STELLARIS 5 Cryo is a confocal light microscope system that helps you to target your area of interest for cryo-electron tomography (CryoET). The STELLARIS 5 Cryo gives you the precision to target reliably, while still offering superior performance you can count on and improved productivity for your experiments.
The precision to target reliably
For correlative workflows, it is crucial to retrieve the exact target coordinates identified in the cryo light microscope because fluorescence signals are not visible in subsequent electron microscope steps.
Targeting is therefore done in the STELLARIS 5 Cryo microscope with open access coordinates for greater system interoperability.
The user-friendly LAS X Coral Cryo software module fulfils these needs, featuring innovative interpolation-based 3D targeting that allows precise positioning of open format coordinate markers in the nanometer scale. Different marker types serving different functions are also available for landmark, lamellae and bead marking.
Performance you can count on
In cryo-electron microscopy, ice thickness must be monitored throughout the workflow, and the earlier it is checked and assessed the better. If the ice is too thick, it can cause issues during the milling stage, whereas if it is too thin, this may affect the stability of the sample.
The STELLARIS 5 Cryo offers two modes for ice checking: a fast camera overview and a confocal reflection mode to determine the ice thickness in 3D.
The Cryo CLEM objective HC PL APO 50x/0.90 has been developed to fulfil the specific needs of cryo light microscopy, offering you the advantages of super-resolution without the need for liquid immersion.
Manufacturer Leica Microsystems
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