SPECTROstar® Omega Absorbance Plate Reader
The SPECTROstar Omega microplate reader is a part of the BMG LABTECH’s family of readers that contain a CCD-based spectrometer for ultra-fast, full spectrum absorbance measurements.
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Very easy to set up and fully automatised
Enzymatic assays
Highly reproducible results and very easy to use
Review Date: 12 Jul 2023 | BMG LABTECH
Bmg makes the best plate readers I have ever used
Analyze effects of compounds on cells
We have the previous version of this product. Our plate reader is super reliable, consistent, and a workhorse. It reads luminescence, fluorescence, absórbanme, and fluorescent polarization. Easy enough to use that we can have interns use it. Love this equipment. Also, BMG are fantastic with customer support. Always willing to help when we want to use new applications with it or the instrument requires service, which is seldom.
Review Date: 17 Apr 2023 | BMG LABTECH
absorbance measurement binding assay
The absorbance plate reader is easy to use and the analysis software linked to it is user friendly, but still can be further improved to fasten the data analysis. The after sales service is also very good. If we have any problem with the analysis software or the reader itself we can easily get in contact with an engineer from BMG and discuss issues. Concerning the value for money, this absorbance plate reader really performs well. To give you an idea: this absorbance plate-reader is really good for binding assay development on microplate based assays. It has very good accuracy measurements, reproducibility and resolution properties. The reading of a 384-well plate is really fast (17min for one entire 384 well plate). I really advise this plate-reader for screening.
Review Date: 8 Oct 2013 | BMG LABTECH
The SPECTROstar Omega microplate reader is a part of the BMG LABTECH’s family of readers that contain a CCD-based spectrometer for ultra-fast, full spectrum absorbance measurements.
In less than 1 second per well, this spectrometer is able to capture an entire UV/Vis absorbance spectrum (220 to 1000 nm) at a resolution of 1 nm. This unique feature allows for absorbance assays to be performed on the SPECTROstar Omega that cannot be performed with other microplate reader technology.
Combined with precise temperature control (up to 65°C), on-board reagent injection, multi-mode shaking capabilities, high reproducibility and a wide dynamic range, the SPECTROstar Omega provides users with unmatched flexibility and sensitivity to perform any absorbance based assay needed.
For cell-based assays, BMG LABTECH’s Atmospheric Control Unit can be added to the SPECTROstar Omega. This optional microprocessor controlled ACU allows users to fully regulate CO2 (0 - 20%) and O2 (1 - 19%) independently within the microplate reader chamber. The ACU provides users with a walk away solution for all cell based assays.
For low volume sample measurements in assays like DNA, RNA, or protein concentration determination, the SPECTROstar Omega can use BMG LABTECH’s LVis Plate. This LVis plate has sixteen microdrop wells that can hold two microliters of sample. By using the LVis plate, researchers will save on precious samples like DNA or RNA.
Due to the modular concept of the Omega series of microplate reader series, the SPECTROstar Omega is entirely upgradeable to a multi-mode microplate reader. Whenever the need for fluorescence, luminescence, or time-resolved fluorescence based experiments arises, the SPECTROstar Omega is easily upgraded to a FLUOstar Omega or even the POLARstar Omega with Simultaneous Dual Emission for fluorescence polarization assays.
Applications:
- 260/280 nm DNA, RNA quantitation
- Protein quantitation (e.g. Bradford, Lowry, BCA)
- Reporter gene assays (e.g. ß-Gal)
- NAD+/NADH
- Cell proliferation and apoptosis
- ELISAs, enzyme immunoassays (e.g. alkaline phosphatase, horseradish peroxidase)
- Enzyme activity assays
- Microbial cell growth
Accessories
- Atmospheric Control Unit (ACU): provides the ideal environment for cell-based assays by regulating O2 and CO2 gas levels in the microplate reader
- Microplate Stacker: optimises microplate handling and increases throughput by providing rapid loading, unloading, restacking, and continuous feeding for up to 50 microplates
- LVis Plate: microplate for low-volume DNA, RNA and protein quantification. It includes 16 micro-drop (2 µL) wells, a cuvette slot and optional performance testing features
- Evaluation Plate: validates the optical functionality of BMG LABTECH microplate readers for quality control and/or qualification (IQ/OQ) purposes
Brochures
SPECTROstar<sup>®</sup> Omega Product Brochure
This product brochure contains information and technical specifications for the SPECTROstar® Omega – a dedicated absorbance microplate reader that can capture either a full absorbance spectrum or up to eight discrete wavelengths simultaneously.
Microplate Readers Application Note Binder
A book of application notes for all assay needs, using BMG LabTech Microplate Readers. This collection showcases the specific capabilities of the different models from the range of BMG LabTech Microplate Readers available.
Absorbance-based Methods for Protein Quantification on BMG LABTECH Instruments
Measuring the protein concentration of liquid samples is a routine analysis in many life science laboratories. Accurate quantification is often a critical step for subsequent analyses such as protein characterization or western blots. Spectrometer-based BMG LABTECH readers capture absorbance spectra or absorbance at discrete wavelengths from 220-1000 nm in less than a second per well and therefore can easily read these assays.
Label-Free SoPRano™ Gold Nano-Rod (GNR) Assays on a Spectrometer-Based Microplate Reader
A label-free SoPRano™ assay from PharmaDiagnostics benefits from fast, full-spectrum analysis found on all BMG LABTECH spectrometer-based microplate readers. The label-free SoPRano™ assay uses the Localized Surface Plasmon Resonance (LSPR) properties of Gold Nano Rods (GNRs) to determine protein-protein interaction such as antibody-antigen binding. The change in LSPR signal upon protein binding can be measured as an absorbance change in the max, which is easily detected with a BMG LABTECH spectrometer-based microplate reader. Read BMG LABTECH’s application note for more details.
Get the best out of your microplate reader
Discover tips and considerations for improving microplate reader measurements. Application specialists, Dr. Ann-Cathrin Volz and Dr. Martin Mangold from BMG LABTECH, discuss the impact of different plate reader parameters on data, and provide empirical evidence of their effect on data quality. They also discuss approaches to optimize microplate reader settings, and share recommendations for using BMG LABTECH's range of microplate readers.
Atmospheric Control for Microplates
This video highlights the Atmospheric Control Unit (ACU), a new accessory for the Omega series of multifunction microplate readers. Two independent intake valves and a microprocessor control panel allow for optimal atmospheric conditions to be maintained inside the microplate reader chamber. The ACU is ideal for creating an environment for all cell based assays, including stem, tumor, mammalian, bacterial, and yeast cells, as well as for mimicking disease states such as hypoxia or anoxia.
