Sigma-Aldrich ® one-part sgRNA and two-part crRNA:tracrRNA systems accelerate genome editing with Cas9 protein, mRNA, or established Cas9 expressing cell lines. Our guides are compatible with a variety of delivery methods including microinjection, electroporation, and lipofection.
Sigma-Aldrich® CRISPR synthetic gRNAs
Merck
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Merck's single guide RNAs are purified using gold standard HPLC methods ensuring the highest quality and lowest toxicity in cellular and transgenic applications.
Selected Applications
Engineer transgenic animal models
Model disease states in immortalized cells
Create isogenic iPS cell lines
Advantages
Guaranteed - Assured cutting for any target sequence including custom designs
HPLC Purified for unparalleled quality
Easy-to-use - Reconstitute gRNA and form RNP complexes within minutes
Customizable – Choose your format, quantity, purification and modification
Fast shipping (usually 3-5 days for crRNA, 7-10 business days for sgRNA)