Status:
ReviewerMember since: 2018
Organization: Garage Tests
No regrets. Buy now!
Application Area: Proteolytic enzyme for downstream LCMS
"Works. Reproducible results. DDA or DIA, this enzyme gets the job done."
Status:
ReviewerMember since: 2021
Organization: University of Oxford
My key tool.
Application Area:Proteome in mitosis
"Very efficient enzyme, missed cleavages happen occasionally."
Status:
ReviewerMember since: 2019
Organization: University of Leicester
Would recommend!
Application Area:Proteomics
"Have use within our service facility for over 10 years and we were always happy with the product and the reproducibility of the data. The aftercare was great!"
Status:
Expert ReviewerMember since: 2016
Organization: Novartis Pharma AG
Enzyme to be trusted
Application Area:Protease for proteomics applications
"The sequencing grade modified trypsin comes in handy glass vials with 5 x 20 micrograms, either lyophilized or frozen. Excellent activity and high batch-to-batch reproducibility. Great both for complex protein mixtures and for individual proteins."
Status:
Advanced ReviewerMember since: 2010
Organization: University of Hamburg
Nice and reliable product
Application Area:proteomics
"It is a nice protease which is resistant to auto-proteolysis and gives digestion to your protein."
Trypsin is a serine protease that specifically cleaves at the carboxylic side of lysine and arginine residues.
The stringent specificity of trypsin is essential for protein identification. Native trypsin is subject to autolysis, generating pseudotrypsin, which exhibits a broadened specificity including a chymotrypsin-like activity. Such autolysis products, present in a trypsin preparation, would result in additional peptide fragments that could interfere with database analysis of the mass of fragments detected by mass spectrometry.
Sequencing Grade Trypsin has been manufactured to provide maximum specificity. Lysine residues in the porcine trypsin have been modified by reductive methylation, yielding a highly active and stable molecule that is extremely resistant to autolytic digestion.
Sequencing Grade Modified Trypsin Features & Benefits:
Manufacturer Promega Corp. | Available Worldwide
4.5 / 5.0 | 5 reviews