Sequencing Grade Modified Trypsin by Promega Corp.

Manufacturer Promega Corp.
4.0
/
5.0
  |  2 reviews
Trypsin is a serine protease that specifically cleaves at the carboxylic side of lysine and arginine residues. The stringent specificity of trypsin is essential for protein identification. Native trypsin is subject to autolysis, generating pseudotrypsin, which exhibits a broadened specificity including a chymotrypsin-like activity. Such autolysis products, present in a trypsin preparation, would result in additional peptide fragments that could interfere with database analysis of the mass of fr... Read more


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Sequencing Grade Modified Trypsin
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Average Rating: 4.0
2 Scientists have reviewed this product

5 out of 5
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money


  • Status:

    Expert Reviewer
  • Member since: 2016

  • Organization: Novartis Pharma AG



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Enzyme to be trusted
Rating: 4.7

  • Application Area: Protease for proteomics applications

"The sequencing grade modified trypsin comes in handy glass vials with 5 x 20 micrograms, either lyophilized or frozen. Excellent activity and high batch-to-batch reproducibility. Great both for complex protein mixtures and for individual proteins."

Review date: 31 Oct 2017 | Sequencing Grade Modified Trypsin
  • Status:

    Advanced Reviewer

  • Member since: 2010

  • Organization: University of Hamburg



  • Ease of use
    4 out of 5
    After sales service
    3 out of 5
    Value for money
    3 out of 5
Nice and reliable product
Rating: 3.3

  • Application Area:proteomics

"It is a nice protease which is resistant to auto-proteolysis and gives digestion to your protein."

Review date: 05 Apr 2017 | Sequencing Grade Modified Trypsin
Trypsin is a serine protease that specifically cleaves at the carboxylic side of lysine and arginine residues.

The stringent specificity of trypsin is essential for protein identification. Native trypsin is subject to autolysis, generating pseudotrypsin, which exhibits a broadened specificity including a chymotrypsin-like activity. Such autolysis products, present in a trypsin preparation, would result in additional peptide fragments that could interfere with database analysis of the mass of fragments detected by mass spectrometry.

Sequencing Grade Trypsin has been manufactured to provide maximum specificity. Lysine residues in the porcine trypsin have been modified by reductive methylation, yielding a highly active and stable molecule that is extremely resistant to autolytic digestion.


Sequencing Grade Modified Trypsin Features & Benefits:

  • TPCK Treatment Followed by Affinity Purification - Elimination of chymotrypsin activity enables distinct and consistent data.
  • Stability - Ensured up to five freeze-thaw cycles.
  • Reliable and Customer-Proven - Referenced in thousands of papers.
  • Alternative Formats - Flexibility depending on experimental design and scope.