SepsiTest™-UMD CE IVD by Molzym GmbH & Co. KG

Microbial DNA enrichment, isolation and PCR detection  


SepsiTest™-UMD CE IVD by Molzym GmbH & Co. KG product image
SepsiTest™-UMD CE IVD
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SepsiTest™-UMD is a CE IVD-marked kit for the culture-independent detection of bacteria and fungi in clinical samples. The detection and identification of bacteria and fungi is based on broad-range 16S and 18S rRNA gene PCR followed by Sanger sequencing. 

The sensitivity of molecular analysis is often limited due to the vast excess of human DNA compared to the low bacterial and fungal load. The human DNA depletion prior to the microbial DNA isolation overcomes this limitation. Reagent-borne contaminations are another risk counteracted with guaranteed DNA-free reagents and consumables provided in SepsiTest™-UMD. The amplification with 40 PCR cycles allows the sensitive detection of even minute amounts of bacterial and fungal DNA and provides a first result after approximately four hours. PCR-positive samples are analysed with Sanger sequencing to identify the pathogens. 

Diverse specimens can be analysed with SepsiTest™-UMD, including different body fluids, swabs and tissue biopsies. 

Benefits:

  • Variety of specimens: body fluids, swabs, tissues
  • Flexible amount of samples
  • Increased sensitivity by depletion of host DNA
  • Contamination-free reagents and consumables
  • Broad-range 16S and 18S rDNA PCR assays

SepsiTest™-UMD is particularly suited for the detection of pathogens in samples from patients pretreated with antibiotics and for fastidious organisms negative in culture. More than 1.300 pathogens have been identified with SepsiTest™-UMD so far, among others, pathogens causing sepsis, bacterial meningitis, pneumonia, joint and wound infections.