Status:
ReviewerMember since: 2013
Organization: Furman University
Application Area: Bullfrog Brain \RNA Isolation
"I have used this product for many applications and have found it to be very versatile. The only thing I have had an issue is with the differing amounts of products that they run out at different times and having to overlap kits. "
Status:
ReviewerMember since: 2013
Organization: Uniformed Services University of the Health Sciences
"It has very high purity RNA isolation. The kit is easy to use and directions that come with the kit do not require troubleshooting. The company was very helpful with any questions and overall customer service was very good. Highly recommend for RNA isolation for applications such as qPCR and sequencing technologies. Extremely reliable. "
Status:
ReviewerMember since: 2013
Organization: Unviersity of California San Francisco
Application Area:Immunofluorescence
"QIAGEN kit provides a very good yield of DNA and plasmid purification. It also provides a very clean plasmid which doesn't require further purification."
Status:
ReviewerMember since: 2013
Organization: Furman University
Application Area:RNA Isolation
"The kit is very easy to use and directions are clear and helpful! It is very useful in our RNA isolation. It is very good value for the money. "
Status:
ReviewerMember since: 2013
Organization: Michigan State University
"It’s easy to use plus has good quality reagents. I believe it is good value for money, quick and versatile to use. It also has good quality read outs. "
Status:
ReviewerMember since: 2013
Organization: University of Ottawa
Application Area:Extraction Protocol
"It is easy to use, quick and efficient. It has resulted in clean products as confirmed by gel imaging. It has also allowed for extremely high yield and with a reading of absorbance at the 260/280 range of 2.0. "
Status:
ReviewerMember since: 2013
Organization: George Mason University
"This product has been vital to our lab. It has helped to make things a lot easier for us when doing these extractions. The ease of use is great."
Status:
ReviewerMember since: 2013
Organization: Furman University
Application Area:RNA isolation
"It is very self-explanatory, well labelled, and easy to use. It has very ambiguous contents of the substances. It’s very versatile in that each is labelled clearly and the most necessary equipment is included, and also well labelled."
Status:
ReviewerMember since: 2013
Organization: John Innes
Application Area:Extraction of viral RNA
"A very simple protocol to follow. I have been using this kit for several years now and have had very few failures. I use many of the Qiagen kits and have found them all to be robust and simple to use."
Status:
ReviewerMember since: 2013
Organization: Mayo Clinic Florida
Application Area:Molecular biology
"There is no substitute, definitely the best RNA purification kit around. For real, it's all I use, however it could be cheaper."
Status:
ReviewerMember since: 2013
Organization: Baylor College Of Medicine
Application Area:isolation of RNA in cancer cell and tissue
"Very good product but so expensive for large scale experiments. Sometimes RNA decay happens. The RNA later reagent does not always work. "
Status:
ReviewerMember since: 2013
Organization: National Cancer Institute
Application Area:cancer
"This kit worked really well for the most part, but I noticed that if I loaded a lot of material onto the column it would clog and I wouldn't get much RNA."
Status:
ReviewerMember since: 2013
Organization: Mayo Clinic
Application Area:cancer research
"Straight forward, and easy to use. Works well for cell line experiments and whole organ tissue. Quick isolation process of tissue as well. Very high RNA yield for my tissue of choice (pancreas)."
Status:
ReviewerMember since: 2013
Organization: Penn State Hershey Colleg Of Medicine
Application Area:Leukemia
"Very easy to use kit. Multi-step process is a bit frustrating, but clearly necessary to complete the process. "
Status:
ReviewerMember since: 2013
Organization: Aveo Pharmaceuticals, Inc.
Application Area:Extraction of RNA for gene expression analysis
"The kit is very easy to use and the RNA extracted is of high quality. Highly recommend the product."
Status:
ReviewerMember since: 2013
Organization: GEORGETOWN UNIVERSITY
Application Area:RNA isolation for qRT-PCR
"This is a very easy to use kit with minimal (if even) optimization required. Does not come with all reagents, other purchases required. Also has specialized kits for high lipid tissue types. Protocol has been optimized for multiple sample types as well."
Status:
ReviewerMember since: 2013
Organization: Nci
Application Area:RNA isolation
"This kit is very user friendly. I have consistently received very high RNA yields. The RNA quality is good as well."
Status:
ReviewerMember since: 2013
Organization: H. Lee Moffitt Cancer Ctr. Res. Inst.
Application Area:Tumor Biology
"The kit is extremely fast and easy to use. Yields high quality RNA from cell culture or tissue samples."
Status:
ReviewerMember since: 2013
Organization: Ucla School Of Dentistry
Application Area:cDNA synthesis
"Isolation of RNA is quite easy and I don't see any DNA contamination because of good columns and buffers. I would like to use this instead of Trizol which is rather risky in terms of purity. I would use other company's product but I am completely satisfied with what I use already."
Status:
ReviewerMember since: 2013
Organization: Univ. Of Mississippi Medical Ctr.
"Hi quality RNA with a good yield, which is better than other products, but a bit pricey when compared to other kits."
Status:
ReviewerMember since: 2013
Organization: Johns Hopkins Univ. School Of Medicine
Application Area:cancer research
"Quick and easy to use. Probably the standard for RNA isolation. The instruction book that comes with the kit is very detailed and answers any question you could have. The resulting RNA is usually very pure."
Status:
ReviewerMember since: 2013
Organization: Universidade Estadual Paulista
Application Area:expression
"This kit is easy to use and the results are great. We save time and money because the RNA extracted is always with an excellent RNA Integrity Number and my qPCR Real time always works."
Status:
ReviewerMember since: 2013
Organization: University Hospital Gasthuisberg- Ku Leuven
Application Area:pcr
"Easy to use, very helpful, high yield and high quality as compared to other kits. The best I have even used and the one I am always suggesting. It is the only one I use."
Status:
ReviewerMember since: 2013
Organization: University Of São Paulo
Application Area:Oncogenetics and Molecular Biology
"A high quality of acid nucleic extracted with this kit. An easy and versatile protocol. I also use the Endo-free Maxi prep to extract plasmids to perform a transfection in mammalian cells. I saw a high quality of the material expressed."
Status:
ReviewerMember since: 2013
Organization: Cemm Research Center For Molecular Medicine Of The Austrian Academy Of Sciences
Application Area:translational research, small molecules investigation
"Since the cost of this and other similar kits from Qiagen has gone a bit higher, we tried to improvise in the lab, trying other possibilities. However, all other protocols and/or kits turned out to be much more difficult to use and with significantly lower yield."
Status:
ReviewerMember since: 2013
Organization: Clovis Oncology, Inc.
Application Area:Oncology
"Kit is very easy to use; simple and fast purification of high-quality RNA from cultured cells. High RNA recovery. I have never had the need to contact Qiagen for any after sales services. The kit isn't as cheap as other kits on the market, but it's a proven product and we're willing to front the extra costs associated with it."
Status:
ReviewerMember since: 2013
Organization: Fundacion Inst. Leloir
Application Area:genomics
"Great RNA quality, yields are not the best from fibrous tumor samples or from biopsies with high adipocyte content."
Status:
ReviewerMember since: 2013
Organization: Genentech, Inc.
Application Area:Fresh Frozen clinical tissue sample prep RNA isolation
"The RNeasy midi kit is very easy and straightforward to use. The SOP is very easy to follow and cost efficient for isolating FF tissue sample prep and isolating RNA (for use in Taqman gene expression assays)."
Status:
ReviewerMember since: 2013
Organization: Nci-frederick
Application Area:RNA purification
"Pros: It's easier to process compared to Trizol or RNA later. High quality of RNA that is produced. Cons: relatively low yield."
Status:
ReviewerMember since: 2010
Organization: UNIV. CENTRAL DEL CARIBE SCHOOL OF MEDICINE
"Very good product. Easy to use, reliable, reproducible, very nice. RNA comes out nice, no need to further purify, good for various applications."
Status:
ReviewerMember since: 2013
Organization: Ut Southwestern Med. Ctr.
Application Area:plasmid preparation
"Very easy to use and get high yield and good quality DNA out. The kit requires less time and is easy to use. You can just follow the protocol to the finish. The system is robust and reliable. You can train a high school student to do it with no problem."
Status:
ReviewerMember since: 2012
Organization: University of Michigan
Application Area:Molecular Biology
"This kit has very easy-to-follow instructions and can be used with a tabletop microcentrifuge, no refrigeration required. As such, it's among the easier reactions that I've run. This kit will generate very clean RNA for use in PCR and qPCR reactions (I haven't used it for other things). I used the kit to isolate RNA from tissue and to clean up TRIzol-extracted RNA samples. For cleanup, this kit always worked and generated high quality RNA if I performed all of the optional steps and repeated the final elution twice after letting the elution buffer sit for 1 minute before spinning. If I did not let the elution buffer sit for 1 minute, my yields were lower. I recommend this kit as a great standard kit for RNA extraction and cleanup, with or without TRIzol; however, I feel that Zymo and Omega Bio-tek now offer kits of the same high quality for a lower price. I have not called Qiagen for after-sales services."
Status:
ReviewerMember since: 2012
Organization: Florida State University
Application Area:Miniprep, cloning
"Qiagen has always provided the most reliable kits in my opinion. They are easy and fast and give the highest concentration compared to other plasmid prep kits."
Status:
ReviewerMember since: 2012
Organization: University Of Alberta
Application Area:mRNA enrichment, mRNA purification after affinity pulldown
"The RNeasy RNA isolation and purification kit is fast and yet robust to purify your RNA prior to cDNA synthesis. It is also quite reliable in maintaining a significant yield in your very limited RNA that interacts with your protein (RNA helicase). Furthermore, it requires 10 minutes and you can immediately proceed with your cDNA synthesis."
Status:
ReviewerMember since: 2009
Organization: University Maryland-College Park
Application Area:TRANSCRIPTOME VERIFICATION
"I use this kit to extract RNA from plant tissue. This kit is really easy to use, it takes about half an hour from tissue homogenation to final RNA elution fro 8 samples. I have never had any problems with degradation. I have always had good results in finding the transcript isoforms. However I do not really like the customer help from Qiagen because they do not think outside the box with problem solving- it is always that solutions are old. But I would recommend this product for total RNA isolation."
Status:
ReviewerMember since: 2012
Organization: University of Maryland
Application Area:Plant cell development and spermatogenesis
"This kit is very easy to use, however the yield for RNA extraction are sometimes lower than expected."
Status:
ReviewerMember since: 2012
Organization: Nih/Niddk
"The instructions are easy to follow even for first time users. It's a great kit for students and people new to DNA purification to use. I love the pre-made buffers because they cut down on work time and variability between samples."
Status:
ReviewerMember since: 2012
Organization: University Central Florida
Application Area:cDNA
"The kit worked well for us. Protocol was easy to follow. Had a little trouble adapting to fit it to our cells though."
Status:
ReviewerMember since: 2012
Organization: Northwestern University
"The RNeasy mini kit is a really convenient way to quickly purify RNA from all cell types and tissues. I have had a lot of luck obtaining high quality RNA from epithelial cells and keratinocytes which I have used successfully in real time PCR reactions. It is quick, easy and fool-proof."
Status:
ReviewerMember since: 2012
Organization: Shanghai Institute Of Biochemistry
Application Area:RNA purification
"This is a very easy to use kit and produces high quality RNA. The produced RNA is suitable for normal RT-PCR or RT-qPCR. It can also be used for mRNA-sequencing and other applications which requires high quality of RNA."
Status:
ReviewerMember since: 2012
Organization: University Of Sci. & Tech. Of China
Application Area:plasmid purification
"This kit is easy to use, we can purify plasmid in a very short time with high quality, but the reagent does quickly run out."
Status:
ReviewerMember since: 2012
Organization: Rmrc/Kribb
Application Area:RNA prep. for human embryonic stem cells
"Very good for the preparation of human embryonic stem cells and human induced pluripotent stem cells."
Status:
ReviewerMember since: 2012
Organization: University of Maryland
Application Area:plant cell biology
"Gives a very high yield, high quality RNA from plant cells. I would highly recommend this product to anyone who is having problems isolating total RNA from plant cells."
Status:
ReviewerMember since: 2012
Organization: UCLA
Application Area:Mammalian Cell RNA Isolation
"This kit was easy to use. As an undergraduate student who does not have a lot of lab experience, I found this kit to be very straight forward to use. I was able to get great results from this product the very first time I used it. Overall, easy to use and good for beginners."
Status:
ReviewerMember since: 2011
Organization: University of California Davis
Application Area:Cell Biology
"Easy to use, manual is clear. Good yield of RNA. Buffers can be bought separately. Consistent results obtained however, in my opinion, it is a bit too expensive."
Fast procedure delivering high-quality total RNA in minutes. Ready-to-use RNA for high performance in any downstream application. Consistent RNA yields from medium amounts of starting material. No phenol/chloroform extraction.