Member since: 2019
Works great for RNAseq, works fine for Riboseq.
Application Area: rRNA depletion prior to RNA sequencing
"We ordered riboPOOL for human samples after Illumina discontinued the RiboZero Gold standalone kit. We needed a rRNA depletion solution that worked not only for RNAseq (and we prefer enzyme-free kits) from total RNA but also for Riboseq on short (17-34 nt) ribosome protected fragments (most of the kits available in the market aren't compatible with this application). We first tested by RTqPCR the depletion of 18S rRNA from total human RNA (MDA-MB-231 cells) and got >2000-fold depletion with equal input of pre- and post-treatment samples. We then sequenced RNA from these samples and got 0.97% of total reads aligning to rRNA. We also tested riboPOOL performance in depleting rRNA fragments from ribosome protected fragment preparations for Riboseq and got 28.07% of reads aligning to rRNA (~2X more than with Illumina's RiboZero Gold in our previous experiments). Overall our experience has been very positive with riboPOOL, the tech support was prompt to reply and the cost of the product is very competitive."
Member since: 2019
Organization: University of Maryland
riboPOOL kit works great for RNA sequencing. I have received good results using this.
Application Area:RNA analysis of human tissue samples and mouse tissue samples. Ribopool kit for RNA samples for sequ
"Works great when you are doing RNA sequencing. riboPOOL kit is species specific. I would highly recommend using this kit. It's user friendly, economic and is sensitive and specific."
Detection of scientifically relevant RNAs by Next-Generation RNA Sequencing (RNA-Seq) is greatly limited by highly abundant ribosomal RNAs (rRNAs) which occupy > 90% RNA-Seq reads. Current rRNA depletion solutions however are often costly and limited to well-studied species.
Ribosomal RNA depletion pools (riboPOOLs) by siTOOLs Biotech is the market's most flexible solution in rRNA depletion. With a diverse selection of Ready-Made riboPOOLs and the ability to create your own Custom riboPOOL, riboPOOLs are ideal for sequencing experts with custom demands and scientists working on rare species.
Composed of high complexity pools of optimally-designed biotinylated DNA probes, riboPOOLs are highly efficient, even outperforming Ribo-Zero (Illumina), depleting ~10% more rRNA while leaving other RNA intact.
For microbiologists, the new Pan-Prokaryote riboPOOL provides universal rRNA depletion for a wide spectrum of microbes and is suited for metagenomic or microbiome samples. Combination riboPOOLs are also available to deplete rRNAs from samples containing multiple species.