Member since: 2019
Organization: Lyon college
Application Area: Click chemistry
"The CW800 alkyne dye along with the revert total protein stain have been exceptional tools for click chemistry approaches to look at nascent protein expression in our lab."
Visualize and quantify the total protein content on Western blot membranes with Revert 700 Total Protein Stain or Revert 520 Total Protein stain. Revert Total Protein Stains provide linear, proportional signal across a broad range of sample concentrations. Use these quick, total protein stains to correct for lane-to-lane variation in loading and transfer of target protein in your quantitative Western blot experiments. Normalizing target protein to a total protein stain like Revert 700 or Revert 520 offers greater quantitative data accuracy. Revert Total Protein Stains do not covalently modify your target protein and therefore do not affect its quantitation. Drop Revert 700 Total Protein Stain or Revert 520 Total Protein Stain right into your existing protocol, without the need for any additional reagents or special gels. The stain will work with the membrane of your choice (PVDF or Nitrocellulose). Stain your blot in just 5 minutes, and image in the 520 or 700 nm channel with an Odyssey® Imaging System.