Member since: 2016
Organization: Immue System Key LTD
Saves time, high quality transfer
Application Area: Western Blot
"Allows rapid transfer on PVDF or Nitrocellulose membrane without ethanol. 10-15 minutes at 25V is enough for small-medium size proteins."
Rapid Transfer Buffer is a simple, single component system for quick and efficient transfer of proteins from SDS-PAGE gels to membranes for Western blotting applications. Transfer is completed in 10 or 20 minutes using a standard semi-dry or wet transfer apparatus, respectively. Rapid Transfer Buffer is a methanol-free, non-hazardous formulation that is compatible with PVDF and nitrocellulose membranes. It is used for transfer of proteins from standard Laemmli gels, commercial pre-cast gels, and AMRESCO’s NEXT GEL™. The transfer efficiency is equivalent to that observed when using a Tris-Glycine-Methanol transfer buffer.