Member since: 2019
Organization: Phylos Bioscience
Quick and simple - good for small amounts of fungi.
Application Area: Extract DNA of fungal plant pathogens and bacterial endophytes
"I have used this product to extract small amounts of fungal pathogens from plant tissues - mostly botrytis and powdery mildews. Simply pressing Scotch tape to the sporulating fungi, then folding up the tape and adding it to the included bead beating tubes, works very well for this application. With the OMNI bead disruptor I use, there is no need to disrupt for 5 minutes on high - I usually do 1 min on level 5. I've also extracted a variety of culturable endophytes from broths or by adding a glob scraped off a plate. Always surprised by how quick and easy this protocol is. Under an hour extraction, even with lots of samples. I have never had problems with downstream applications (PCR, qPCR, cloning, sequencing). Yields are good for both these types of samples, but I would prefer a slightly higher volume than the 20ul this kit yields without sacrificing on concentration. I'll have to try the miniprep. Recommended."