Member since: 2013
Organization: University Of Colorado Boulder
Application Area: In Situ Hybridization
"You do need specific equipment, such as particular centrifuge sizes or the vacuum manifold. If you have that it works well but trying to get around that results in lower yields."
Member since: 2012
Organization: Stanford University
"This worked well for me, I got very reasonable yields and it wasn't difficult to use. I don't have a good benchmark to compare to the Qiagen kit though."
Member since: 2010
Organization: Miami University
Application Area:DNA isolation
"Instructions are very easy to practice. Even not so skilled undergraduates can do DNA isolations. Midi-prep kits give us a good yield almost all the time."
Member since: 2012
Organization: Colorado State University
"The kit is definitely quick and easy to use - the 2mL culture size is handy. The disadvantage of PureYield compared to Qiagen kits is the yield, 7.5ug of plasmid max versus 30ug for Qiagen."
The system provides a rapid method for purification of 100–200μg of plasmid DNA from 50ml bacteria culture. Plasmid DNA can be purified in as little as 30 minutes with the vacuum protocol, greatly reducing the time spent on purification compared to silica resin or other membrane-column methods. An alternative protocol allows purification of over 400μg of high-copy-number plasmid from 250ml of E. coli culture.
The PureYield™ Plasmid Midiprep System incorporates a unique Endotoxin Removal Wash designed to remove substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA. Removal of contaminants improves the robustness of sensitive applications such as eukaryotic transfection, in vitro transcription and coupled in vitro transcription/translation (e.g., TnT® Quick Coupled Transcription/Translation System).
Purification is achieved without isopropanol precipitation of purified plasmid DNA or extensive centrifugation, providing rapid purification from a single method. The system has been designed for use with centrifugation or vacuum (e.g., the Vac-Man® Laboratory Vacuum Manifold).
PureYield™ Plasmid Midiprep System Features & Benefits: