PureLink™ HiPure Plasmid Filter Maxiprep Kit by Thermo Fisher Scientific

Manufacturer Thermo Fisher Scientific  |   Model: K210016  |  Available Worldwide
4.0
/
5.0
  |  1 reviews
The PureLink™ HiPure Plasmid Filter Maxiprep kit is designed to isolate plasmid DNA of the highest purity


PureLink™ HiPure Plasmid Filter Maxiprep Kit by Thermo Fisher Scientific product image
PureLink™ HiPure Plasmid Filter Maxiprep Kit

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Average Rating: 4.0
1 Scientist has reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
2 out of 5
Value for money


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    Reviewer
  • Member since: 2019

  • Organization: University of Liverpool



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    2 out of 5
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Good detailed information online for troubleshooting
Rating: 4.0

  • Application Area: DNA extraction

"Extremely easy to use (beginner/ undergrad/research level) with simple detailed instructions and high success rates. Good detailed information online for troubleshooting. Multi-use product and relatively quick. Only downside is the expense at £200. "

Review date: 22 Nov 2019 | PureLink™ HiPure Plasmid Filter Maxiprep Kit

The PureLink® HiPure Plasmid Filter Maxiprep Kit is designed to isolate plasmid DNA of the highest purity from 100 to 500 mL of an overnight bacterial culture. The PureLink® HiPure Plasmid Filter Kits include HiPure Filter Columns, which combine filtration for clarification of the bacterial lysate with anion-exchange resin purification of plasmid DNA, in a single unit. The resulting plasmid DNA is of a quality equivalent to that obtained by purification with two passes through cesium chloride gradients—the most rigorous method for DNA purification. Purify high-quality DNA in less than two hours for transfections and other applications with no need for additional steps to remove contaminants like RNA, proteins, or endotoxins. Additionally, the elimination of phenol, chloroform, ethidium bromide, and cesium chloride from the protocol minimizes exposure to, and disposal of hazardous materials. The PureLink® HiPure Plasmid Filter Kits provide:

• Simple protocols—bacterial lysate clarification without centrifugation
• Fast results—save time by purifying plasmid DNA in less than two hours
• High yields—up to 200 µg for midipreps and up to 850 µg for maxipreps (see table)
• Reliable performance—the high-quality plasmid DNA purified with these kits is suitable for a wide variety of applications

How it works
The addition of a HiPure Filter Column to the PureLink® HiPure Plasmid Kits provides rapid clearing of the bacterial lysate without the need for centrifugation (see figure). The lysate filtration cartridge is integrated into the DNA-binding column for a combined step of lysate clarification and plasmid DNA binding directly to the anion-exchange resin. A single column wash removes impurities such as RNA, proteins, metabolites, and other low molecular weight molecules; the ultrapure plasmid DNA is then eluted with a high-salt buffer. The DNA is then desalted and concentrated with an isopropanol precipitation step, then collected by centrifugation. The entire protocol can be completed in about two hours.

Product Overview

PureLink™ HiPure Plasmid Filter Maxiprep Kit by Thermo Fisher Scientific product image

PureLink™ HiPure Plasmid Filter Maxiprep Kit

Manufacturer Thermo Fisher Scientific  |  Available Worldwide

4.0 / 5.0 | 1 reviews