Pico Methyl-Seq™ Library Prep Kit by Zymo Research

Manufacturer Zymo Research  |   Model: D5455
4.5
/
5.0
  |  2 reviews
The Pico-Methyl-Seq™ Library Prep Kit features a unique post-bisulfite library preparation workflow for Whole Genome Bisulfite Sequencing (WGBS).


Pico Methyl-Seq™ Library Prep Kit by Zymo Research product image
Pico Methyl-Seq Library Prep Kit
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Average Rating: 4.5
2 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2021

  • Organization: University of Michigan



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Easy to use for problematic samples.
Rating: 4.7

  • Application Area: Global DNA methylation profiling

"It is an easy to use product with very simple and detailed protocol. Usually library preps are an over-complicated process, especially in case of DNA methylation analysis. It has the advantage of generating a library from very small amount of DNA. It also includes all the required primers for sequencing, although it needs to be updated to support dual barcoding. With most problematic samples the mapping efficiency obtained using this kit is a bit concerning because it might fall below 30%, it can be improved though."

Review date: 28 Jul 2021 | Pico Methyl-Seq™ Library Prep Kit
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: University of Rhode Island



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Good results with modifications.
Rating: 4.3

  • Application Area:Library prep for whole genome bisulfite sequencing

"This works for non-model organisms such as corals. However, we had to make a few modifications which included increasing the volume of the library amp master mix. Unfortunately, you cannot purchase this separately at the moment, so the only way to get more of this master mix is by purchasing an entire kit (which is very expensive!)."

Review date: 21 May 2021 | Pico Methyl-Seq™ Library Prep Kit

The Pico Methyl-Seq™ Library Prep Kit provides a streamlined workflow for making WGBS libraries. Input DNA is randomly fragmented during the initial bisulfite treatment step followed by three rounds of amplification with uniquely designed primers. The procedure can accommodate as little as 10 pg input DNA (including that derived from FFPE samples), making it ideal for methylation analysis of precious, limited, and target-enriched samples.

Product Overview

Pico Methyl-Seq™ Library Prep Kit by Zymo Research product image

Pico Methyl-Seq™ Library Prep Kit

Manufacturer Zymo Research

4.5 / 5.0 | 2 reviews