Status:
ReviewerMember since: 2022
Organization: University of Rhode Island
Very easy to use and modify/troubleshoot
Application Area: Library prep for whole genome bisulfite sequencing
"Very easy to use and modify/troubleshoot"
Status:
ReviewerMember since: 2022
Organization: University of Rhode Island
Good results and would purchase again.
Application Area:Library prep for whole genome bisulfite sequencing
"It was relatively easy to use and troubleshoot. Some of the master mixes did run out quickly though, so more kits had to be purchased to accommodate the missing volume."
Status:
ReviewerMember since: 2021
Organization: University of Michigan
Easy to use for problematic samples.
Application Area:Global DNA methylation profiling
"It is an easy to use product with very simple and detailed protocol. Usually library preps are an over-complicated process, especially in case of DNA methylation analysis. It has the advantage of generating a library from very small amount of DNA. It also includes all the required primers for sequencing, although it needs to be updated to support dual barcoding. With most problematic samples the mapping efficiency obtained using this kit is a bit concerning because it might fall below 30%, it can be improved though."
Status:
ReviewerMember since: 2021
Organization: University of Rhode Island
Good results with modifications.
Application Area:Library prep for whole genome bisulfite sequencing
"This works for non-model organisms such as corals. However, we had to make a few modifications which included increasing the volume of the library amp master mix. Unfortunately, you cannot purchase this separately at the moment, so the only way to get more of this master mix is by purchasing an entire kit (which is very expensive!)."
The Pico Methyl-Seq™ Library Prep Kit provides a streamlined workflow for making WGBS libraries. Input DNA is randomly fragmented during the initial bisulfite treatment step followed by three rounds of amplification with uniquely designed primers. The procedure can accommodate as little as 10 pg input DNA (including that derived from FFPE samples), making it ideal for methylation analysis of precious, limited, and target-enriched samples.