pGEM®-T Easy Vector Systems by Promega Corp.

Manufacturer Promega Corp.
4.7
/
5.0
  |  5 reviews
The pGEM®-T Easy Vector Systems are convienent systems to clone PCR products. They offer all of the advantages of the pGEM®-T Vector Systems with added convieneice of recognition sites for EcoRI and NotI flankin the insertion site. Thus, several options exist to remove the desired insert DNA with a single restriction digestion. The pGEM(R)-T Easy Vectr System II contains JM109 Competent Cells in addition to all pf the pGEM(R)-T Easy Vector System I components.pGEM®-T Easy Vector Systems Feature... Read more


pGEM®-T Easy Vector Systems by Promega Corp. product image
pGEM®-T Easy Vector Systems
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Average Rating: 4.7
5 Scientists have reviewed this product

5 out of 5
Ease of use
4 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2019

  • Organization: Miss



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Our 'go to' for cloning
Rating: 5.0

  • Application Area: Cloning metastasis genes for expression and sequencing analysis

"The pGEM-T easy vector system is one of the best products we use for cloning. It has high efficiency for the genes we work with and purchase the system with the competent cells as we are guaranteed results. The step by step protocol is easy to follow and is quick. We have also managed to reproduce the results and use the same methods for many genes."

Review date: 27 Jun 2019 | pGEM®-T Easy Vector Systems
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University of Cambridge



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Great result, very quick and easy to use.
Rating: 4.7

  • Application Area:Checking PCR

"Very easy to use, clear information and instructions are super easy to follow. Gave exactly the results that were expected, and very simple to use to check PCR products. Would not use any other product."

Review date: 27 Jun 2019 | pGEM®-T Easy Vector Systems
  • Status:

    Advanced Reviewer

  • Member since: 2017

  • Organization: Punjab Agricultural University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great product in cloning
Rating: 5.0

  • Application Area:Cloning and Sequencing

"The pGEM-T easy vector system is one of the best product in cloning with high efficiency. I prefer to buy them along with competent cells, even 1/4 of the recommended amount of competent cells works well. I tried many cloning kits but feel this is the best one in term of repeatability of results"

Review date: 21 Jul 2018 | pGEM®-T Easy Vector Systems
  • Status:

    Advanced Reviewer

  • Member since: 2017

  • Organization: Punjab Agricultural University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
I am fond of this product
Rating: 5.0

  • Application Area:Cloning and sequencing

"This is really the product which has high efficiency and reproducibility. I suggest buying it alongside competent cells. It works great with very little chance of calibration"

Review date: 27 Feb 2018 | pGEM®-T Easy Vector Systems
  • Status:

    Reviewer

  • Member since: 2010

  • Organization: University of Murcia



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
Rating: 4.0

Review date: 02 Feb 2010 | pGEM®-T Easy Vector Systems
The pGEM®-T Easy Vector Systems are convienent systems to clone PCR products.

They offer all of the advantages of the pGEM®-T Vector Systems with added convieneice of recognition sites for EcoRI and NotI flankin the insertion site. Thus, several options exist to remove the desired insert DNA with a single restriction digestion. The pGEM(R)-T Easy Vectr System II contains JM109 Competent Cells in addition to all pf the pGEM(R)-T Easy Vector System I components.


pGEM®-T Easy Vector Systems Features & Benefits:

  • Flexibility - The multiple cloning site is flanked by restriction enzyme sites for BstZI, NotI and EcoRI, allowing three options to remove the insert with a single digest.
  • Rapid Ligation - The 2X Rapid Ligation Buffer provided allows reactions to be completed in 1 hour at room temperature.
  • Blue/White Screening - T7 and SP6 RNA polymerase promoters flank a multiple cloning region within the α-peptide coding region for β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be directly identified by color screening on indicator plates.
  • f1 Origin of Replication - Allows preparation of single-stranded DNA.