Member since: 2016
Organization: University of Malaya
Direct protocol, simple machine, versatile software, more than one publishable output
Application Area: Sequencing of DNA samples
"With the long-read sequences generated from the machine, I am able to get the complete genomic sequences of my bacterial genome and also the DNA methylation profile of my samples. The library prep is straight-forward and the sequencing part is automated. With its Iso-Seq protocol, I have a longer read thus a higher level of confidence in analyzing my sample. Besides, I can perform a hybrid assembly, using PacBio long reads as the scaffold and Illumina short reads for its accuracy and coverage. With this, I able to sequence a huge area of the repetitive region of the eukaryotic genome. Users can use the PacBio online interface for data transfer and data analysis. The online analysis portal is easy to use, and there is even a community-based forum for PacBio so that the users can share protocols. The consumables are relatively more expensive but the amount of information I can obtain from the sequences upon analysis is very helpful for my research."
Member since: 2016
Definitely recommend PacBio to anyone looking at small genomes,
Application Area:DNA sequencing
"Occasionally hits a stumbling block, but most of the time the instrument runs like a dream with little optimization required. Results are highly accurate with average read lengths over 16kB and the longest reads over 60kB consistently. Lives up to its promises. Definitely recommend PacBio to anyone looking at small genomes, repetitive regions, high GC content. I'm sure the Sequel will perform just as well."
Member since: 2014
Organization: University Hospital Essen, Germany
Application Area:NG Sequencing service
"Sample preparation was a bit difficult it took quite long until results were ready."
Member since: 2013
Organization: National Bureau of Fish Genetic Resources
Application Area:DNA Sequencing High Throughput
"It is convenient to use. It high read lengths with increased and high accuracy. It reads repeat regions and gives real time sequencing. It gives map-able data for use without having to sort out the quality data; hence the people with less bioinformatics capabilities can make their research ideas on sequencing into reality and by investing lesser time than PCR based sequencing systems."