Oligo Pro II System
The Agilent Oligo Pro II system is a UV-based parallel capillary electrophoresis platform for the rapid analysis of ssDNA and ssRNA oligomer purity.
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Wonderful for rapid diagnosis
DNA RNA rapid identification
Rapid identification and easily used for viruses.
Review Date: 23 Dec 2021 | Agilent Technologies
It's simple to set up and use and gives great results.
Electrophoresis
The longer capillaries allow for greater resolution than the LabChip or the BioAnalyzer.
Review Date: 23 Dec 2021 | Agilent Technologies
Accurate determination of oligonucleotide purity is essential for numerous sensitive applications, from PCR to probe hybridizations. The Agilent Oligo Pro II provides users with a dynamic oligonucleotide QC solution, capable of determining the purity of 12, 24, or 96 oligomers in parallel.
The Oligo Pro II provides single nucleotide resolution (n-1) for oligonucleotides up to 60-mer in length, without the use of intercalating dyes or fluorescent probes, directly measuring the purity of ssRNA or ssDNA samples with UV detection. Furthermore, due to UV detection and the use of denaturing gels, results are free of sequence and size-dependent variations, providing users with reliable results, no matter what.
Features & benefits:
- Single nucleotide resolution – Determine the purity of oligonucleotide synthesis products with n-1 resolution through 60-mer
- Fast separations – Complete 12, 24, or 96 separations in approximately 1 hour
- Direct detection of oligonucleotides – UV detection enables the accurate analysis of ssRNA and ssDNA samples without the use of fluorescent probes or intercalating dyes
Brochures
Agilent automated electrophoresis portfolio brochure
Whether performing low- or ultra-high-throughput testing, Agilent Technologies' automated electrophoresis instrument portfolio delivers reliable, objective assessments of sample integrity, concentration, and fragment size. Explore the complete portfolio of automated electrophoresis instruments, application-specific assays, intuitive software, and expert technical services that provide flexible solutions for robust and objective sample quality assessment.
Performance characteristics of the Agilent Oligo Pro ll System
In this application note, Agilent provides experimental data relating to the performance characteristics of the Agilent Oligo Pro II System. This automated capillary electrophoresis instrument is designed for high-throughput separation and purity analysis of the single-stranded DNA (ssDNA) and RNA oligonucleotides (oligos). As oligos are synthesized, small amounts of impurities can be introduced at each step. Different synthesis methods and purification efficiencies can impact the quality of the sample, requiring assessment of the oligo to ensure it is of the purity level needed for downstream applications such as PCR, gene assembly, and microarray/hybridization assays.
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