Octet® R2 BLI System

In this application note, Sartorius demonstrates the power of using the Ambr® 15 Cell Culture system with an integrated Vi-CELL XR and Ambr® Analysis Module together with an Octet® to identify optimum yield conditions in media screening and process optimization experiments.

This application note describes the method setup process for a successful protein-small molecule binding interactions analysis on Octet® R2, R4, R8, RH16, and RH96 instruments. Discover tips on best practices for the preparation of optimal reagents for small molecule binding studies and data analysis including data referencing procedures.

This application note presents a vaccine potency assay performed using Octet systems, in which samples are analyzed in their natural state without the use of detergents. Octet systems are capable of analyzing whole virus, split virions and recombinant HA vaccine samples.

Your research should be guided by your imagination, not by the limitations of your label-free system. In this brochure, Sartorius introduces the Octet® platform's, with flexible, open-format design and comprehensive menu of biosensors letting you attack research challenges in unique new ways.

In this infographic, Sartorius presents a label-free alternative for characterizing protein-protein and drug-protein interactions in standard microtiter plate format and helps you choose an Octet® system tailored to your particular requirements.

In this application note, Sartorius showcase an approach to screen for mannose content in human mAb samples utilizing the Octet® GlyM Kit.

In this brochure, ForteBio showcases the Octect Platform for real-time, label-free analysis for determination of affinity, kinetics, and antibody/protein concentration. Assays are performed using biosensors, reagent and assay kits in 96- and 384-well microplates making it a significantly easier and faster technique than other comparative label-free technologies.

The Fc region of human IgG contributes to a number of beneficial biological and pharmacological characteristics of therapeutic antibodies. One of the most important is prolonging plasma halflife, due to its unique, pH-dependent interaction with the neonatal Fc-receptor (FcRn). Because altered FcRn binding can increase or decrease serum half-life of Fc-containing therapeutics, thereby impacting drug efficacy, FcRn binding interactions are increasingly being assessed at multiple stages of biologic drug development. In this application note, assay design and best practices for FcRn-IgG kinetic analysis on the Octet system will be discussed, as well as considerations for assay optimization, data acquisition, curve fitting, and analysis of results.

This application note describes a study in which several BAC’s CaptureSelect affinity ligand were conjugated to biotin. Results showed that ForteBio’s Streptavidin (SA) biosensors demonstrated excellent compatibility with the biotinylated CaptureSelect affinity ligands, enabling a whole range of novel selectivities for antibody analytics on the octet platform. It concluded that biotinylated CaptureSelect affinity ligands demonstrated excellent compatibility with ForteBio’s streptavidin (SA) enabling a whole range of novel selectivities for antibody detection, quantitation, and characterization on the Octet platform.

This application note describes how the Octet instrument family – a real-time, label-free system – can be used to analyze the kinetics of protein-protein and protein-small molecular interactions. It describes tips and tricks for obtaining the best possible data which include optimizing the target of immobilization and analyte binding. It concludes that carefully optimization will allow the Octet system to yield excellent kinetic data.

Assessing AAV vector quality in gene therapy manufacturing is crucial, but the current gold standard, serotype-specific ELISA, has its drawbacks — time-consuming and prone to matrix effects. In this webinar, Dr. Yoann Saucereau from ExcellGene reveals how a label-free approach not only cut down on complexities in biological matrices but also enabled a quick, routine method. Now, ExcellGene effortlessly analyzes up to 96 samples in under 30 minutes for efficient AAV platform development.

Key learning objectives

• Explore how suitable QC workflows are established in AAV development and manufacturing
• Discover the critical role of assay design and development to determine correct binding kinetics, affinity values or analyte concentrations
• Consider the optimization and evaluation of an AAV titer assay in comparison to Octet® AAVX Biosensors

Who should attend?

• Lab researchers

Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.

The Sartorius Octet® bio-layer interferometry (BLI) system allows researchers to analysis protein-protein and protein-small molecule interactions. In this video, Sartorius introduces the Octet® R series that provides a modular and scalable solution for molecular screening in 96-well microplates.

In this video, Sartorius demonstrates the range of applications bio-layer interferometry (BLI) has in biomolecular analysis workflows and the drug discovery pipeline, including the benefits of the Octet® BLI systems across the entire workflow.

In this video, Sartorius presents the evolution of the Octet® bio-layer interferometry (BLI) system, providing researchers with a label-free approach to monitoring biomolecular interactions, for over 15 years.

Learn why so many choose ForteBio Octet® Systems in these customer testimonials.

Rapidly determine affinity, kinetics, and specificity of protein-protein and protein-small molecule interactions and measure protein concentrations in crude samples