Member since: 2019
Organization: Harvard University
Great results, similar to acoustic shearing but easier!
Application Area: DNA fragmentation and library preparation
"High quality results, similar to more cumbersome acoustic shearing. I consulted NEB customer service for a custom application of this product, and they were helpful as always."
NEBNext dsDNA Fragmentase contains two enzymes, one randomly generates nicks on dsDNA and the other recognizes the nicked site and cuts the opposite DNA strand across from the nick, producing dsDNA breaks. The resulting DNA fragments contain short overhangs, 5´-phosphates, and 3´-hydroxyl groups.
NEBNext dsDNA Fragmentase does not introduce any detectable bias during the sequencing library preparation and no difference in sequence coverage is observed using the two methods
NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. The reagents included within these master mix sets must pass rigorous quality control standards and are lot controlled, both individually and as a set of reagents.
NEBNext® dsDNA Fragmentase® Includes: