NanoBRET™ Target Engagement HDAC Assays by Promega Corp.

Manufacturer Promega Corp.  |  Available Worldwide
Directly Measure HDAC-Test Compound Affinity and Residence Time in Live Cells


NanoBRET™ Target Engagement HDAC Assays by Promega Corp. product image
NanoBRET™ Target Engagement HDAC Assays
Request Pricing

Receive your quote directly from the manufacturer.



0 Scientists have reviewed this product


Write the First Review

No Reviews

NanoBRET™ Technology for Target Engagement

The NanoBRET™ Target Engagement (TE) Assay measures compound binding at select target proteins in intact cells, in real time. The NanoBRET™ TE Assay uses four key components: An expressed cellular target protein that is fused to the bright NanoLuc® luciferase; a cell-permeable fluorescent tracer that specifically binds to the target protein; a substrate for NanoLuc® luciferase; and a cell-impermeable inhibitor for NanoLuc® luciferase.

The assay uses bioluminescence resonance energy transfer (BRET), achieved by transferring the luminescent energy from NanoLuc® luciferase to the fluorescent tracer that is bound to the target protein-NanoLuc® fusion. This energy transfer makes it possible to directly measure compound binding affinity as well as compound-target residence time.

Compounds that are applied to the cells and specifically engage the intracellular target protein-NanoLuc® fusion will result in a decrease in BRET. To ensure accurate assessment of intracellular target engagement, a NanoLuc® inhibitor is used to mitigate any extracellular NanoLuc® signal that may arise from cells compromised during handling, while not adversely affecting NanoLuc® luciferase expressed within healthy living cells

Product Overview

NanoBRET™ Target Engagement HDAC Assays by Promega Corp. product image

NanoBRET™ Target Engagement HDAC Assays

Manufacturer Promega Corp.  |  Available Worldwide

Be the first to review this product