NanoBRET™ TE Intracellular Kinase Assay by Promega Corp.

Manufacturer Promega Corp.  |   Model: N2520  |  Available Worldwide
4.6
/
5.0
  |  21 reviews

Measure Kinase-Ligand Affinity in Live Cells The NanoBRET™ Target Engagement (TE) Intracellular Kinase Assay measures compound binding at select target kinases in intact cells. The assay is based on the NanoBRET™ System, an energy transfer technique designed to measure molecular proximity in living cells. The NanoBRET™ TE Assay measures the apparent affinity of test compounds by competitive displacement of the NanoBRET™ tracer, reversibly bound to a NanoLuc® lucife... Read more


NanoBRET™ TE Intracellular Kinase Assay by Promega Corp. product image
NanoBRET™ TE Intracellular Kinase Assay
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Average Rating: 4.6
21 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


Great, innovative assay!
Rating: 5.0

  • Application Area: Drug discovery and pharmacology validation

"The NanoBRET system is a great application of FRET technology to produce an in-cell assay for drug-kinase binding, which is helpful in the development of inhibitors."

Review date: 17 Apr 2018 | NanoBRET™ TE Intracellular Kinase Assay
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: UNC-CH



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Essential and field-changing technology.
Rating: 5.0

  • Application Area:Running kinase assays in our lab

"Easy to use, high quality and reproducible results. Highly recommended to others!"

Review date: 17 Apr 2018 | NanoBRET™ TE Intracellular Kinase Assay
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: UNC Eshelman School of Pharmacy



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
The NanoBRET cellular kinase engagement assay is poised to set a new standard!
Rating: 4.7

  • Application Area:Kinase probe discovery in open access science

"I am thrilled with the NanoBRET cellular target engagement assay for kinases. It is easy to use, highly relevant and reproducible, and wonderfully supported by Promega."

Review date: 17 Apr 2018 | NanoBRET™ TE Intracellular Kinase Assay
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: UNC Chapel Hill



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Another highly useful product from the group at Promega !
Rating: 5.0

  • Application Area:To better understand target engagement of kinase inhibitors

"Innovative and effective use of bioluminescent technology to help us understand how compounds interact with kinases in cells. Methodology well worked out, straightforward to use and interpret data - great tool to help study target engagement !"

Review date: 17 Apr 2018 | NanoBRET™ TE Intracellular Kinase Assay
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: UNC



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great product. Innovative company
Rating: 5.0

  • Application Area:Kinase assays

"Easy to use. High quality data. Reproducible. Good customer support"

Review date: 17 Apr 2018 | NanoBRET™ TE Intracellular Kinase Assay
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: Theravance Biopharma



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Great assay system
Rating: 4.3

  • Application Area:This assay helped a lot for high throughput screening for kinase protein inhibitor.

"Can easily generate IC50 - HTP compound screening - Easy to use"

Review date: 17 Apr 2018 | NanoBRET™ TE Intracellular Kinase Assay
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: The Institute of Cancer Research



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    3 out of 5
A very exciting technique, I think it will be used more and more in the future
Rating: 3.7

  • Application Area:TE Assay

"It gave good robust results and it was easy to use. I would recommend it."

Review date: 17 Apr 2018 | NanoBRET™ TE Intracellular Kinase Assay
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: Scarab Genomics



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Great
Rating: 4.3

  • Application Area:Drug discovery

"Works well, easy to use. Great technical services department!"

Review date: 16 Apr 2018 | NanoBRET™ TE Intracellular Kinase Assay
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: Roche



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Fantastic tool. Let your creativity loose to discover ways it can be utilized.
Rating: 4.7

  • Application Area:Target engagement

"NanoBRET has addressed a long-standing issue in small molecule drug discovery: what target occupancy is achieved in the complex intracellular environment."

Review date: 16 Apr 2018 | NanoBRET™ TE Intracellular Kinase Assay
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: Reagent Genie Ireland LTD



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Excellent
Rating: 5.0

  • Application Area:Kinase screen

"Excellent product that was very easy to use. Yes, definitely recommend."

Review date: 16 Apr 2018 | NanoBRET™ TE Intracellular Kinase Assay


  • Status:

    Reviewer

  • Member since: 2018

  • Organization: Howard University



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Best research product of the last year.
Rating: 4.7

  • Application Area:cell signaling

"very easy to use and very effective in determining cell signaling."

Review date: 16 Apr 2018 | NanoBRET™ TE Intracellular Kinase Assay
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: Goethe University / Structural Genomics Consortium Frankfurt



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
This will be the next profiling technology of future drug discovery programs!
Rating: 5.0

  • Application Area:Focus: Protein kinase inhibitor profiling in living cells at cellular [ATP] using full-length constr

"Very easy to use due to detailed protocols from the manufacturer. Gained insights in kinase inhibitor affinities in an in vivo context at cellular [ATP] using full-length constructs. Assay z' is usually very high because of little variability between replicates. Advantage of readout over FRET: no photobleaching during data collection because of the bioluminescence readout. The instrument enables in vivo kinetic measurements in intact human cells. Would recommend."

Review date: 16 Apr 2018 | NanoBRET™ TE Intracellular Kinase Assay
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: galapagos



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Great and innovative technology
Rating: 4.3

  • Application Area:drug discovery

"This technology enables us to test binding properties of small molecules to numerous targets in a less or more generic way. The technology was easily implemented in our screening cascade without excessive assay development. I would strongly recommend to researchers in the area of drug discovery to consider this technology."

Review date: 16 Apr 2018 | NanoBRET™ TE Intracellular Kinase Assay
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: C4 Therapeutics



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great assay for measuring compound binding in cells.
Rating: 5.0

  • Application Area:Measruing the cpd binding of the target protein in cells.

"The assay development process was very straight forward and Promega gave great technical support during the process. The assay itself was easy to use with high-quality results and we have implemented this assay to our current profiling workflow for 6 months now."

Review date: 16 Apr 2018 | NanoBRET™ TE Intracellular Kinase Assay
  • Status:

    Reviewer

  • Member since: 2012

  • Organization: Lead Discovery Center GmbH



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Great product, gives new capabilities for hard to express kinase targets.
Rating: 4.3

  • Application Area:The NanoBRET™ TE intracellular kinase assay is used for the characterization of compounds.

"The NanoBRET™ TE intracellular kinase assay protocols are easy to establish and give excellent data reproducibility. I can fully recommend the assay to others."

Review date: 22 Mar 2018 | NanoBRET™ TE Intracellular Kinase Assay
  • Status:

    Reviewer

  • Member since: 2009

  • Organization: LDC



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Great Product.
Rating: 4.3

  • Application Area:Kinase Biology.

"Great Product. Very good support."

Review date: 22 Mar 2018 | NanoBRET™ TE Intracellular Kinase Assay
  • Status:

    Reviewer

  • Member since: 2011

  • Organization: AstraZeneca



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Enabling technology for our applications.
Rating: 4.7

  • Application Area:Target engagement and cell-kinetics

"The technology has been enabling for investigation of target engagement and preliminary studies of in cell-kinetics. High quality results are readily obtainable for the targets we have studied to date and the support from the R&D team at Promega has been outstanding."

Review date: 22 Mar 2018 | NanoBRET™ TE Intracellular Kinase Assay
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: The University of North Carolina at Chapel Hill



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
The NanoBret has proven to be a key technology for our drug discovery efforts!
Rating: 5.0

  • Application Area:I am using this product to evaluate target engagement of protein kinases involved in ER stress.

"Our studies are demonstrating how helpful the NanoBret technology can be for our research. It is easy to transfect cells with this reagent and the resulting data were of high quality."

Review date: 22 Mar 2018 | NanoBRET™ TE Intracellular Kinase Assay
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Ctcc



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Great instrument.
Rating: 4.3

  • Application Area:Lead Discovery

"It is very easy to use, time saving, and always generates reliable data."

Review date: 22 Mar 2018 | NanoBRET™ TE Intracellular Kinase Assay
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Galapagos NV



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Great technology, easy to use!
Rating: 4.0

  • Application Area:Hit profiling.

"NanoBRET kinase assays have been proven to be useful in most cases to add in a hit profiling cascade between biochemical assay and a cellular activity assay. The technology is user friendly."

Review date: 21 Mar 2018 | NanoBRET™ TE Intracellular Kinase Assay
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Bristol-Myers Squibb



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Fantastic product - completely transformed our workflow!
Rating: 5.0

  • Application Area:Single Cell ICP-MS analysis for nanotoxicity and during drug development.

"NanoBRET is a super easy-to-use kit, and we get very clean high quality data every time."

Review date: 31 Jan 2018 | NanoBRET™ TE Intracellular Kinase Assay

Measure Kinase-Ligand Affinity in Live Cells

The NanoBRET™ Target Engagement (TE) Intracellular Kinase Assay measures compound binding at select target kinases in intact cells. The assay is based on the NanoBRET™ System, an energy transfer technique designed to measure molecular proximity in living cells. The NanoBRET™ TE Assay measures the apparent affinity of test compounds by competitive displacement of the NanoBRET™ tracer, reversibly bound to a NanoLuc® luciferase-kinase fusions in cells. 

How it Works
Using the NanoBRET™ TE Kinase Assay, a fixed concentration of tracer (K-4 or K-5) is added to cells expressing the desired NanoLuc® kinase fusion to generate a BRET signal. Introduction of competing compounds results in a dose-dependent decrease in NanoBRET™ signal, which allows quantitation of the intracellular affinity of the target protein for the test compound.

Product Overview

NanoBRET™ TE Intracellular Kinase Assay by Promega Corp. product image

NanoBRET™ TE Intracellular Kinase Assay

Manufacturer Promega Corp.  |  Available Worldwide

4.6 / 5.0 | 21 reviews