Liver enzymes metabolize xenobiotics and endogenous metabolites by glucuronidation, making downstream quantification difficult. Most urine analysis and drug-testing workflows remove the glucuronide using β-glucuronidase. Importantly, the optimized activation temperature for β-glucuronidase impacts the suitability of a specific formulation for any given testing lab. Historically, our most popular recombinant β-Glucuronidase reagents have an optimal temperature of 70°C, which is perfect for the traditional testing lab workflow.
However, enzymes for workflows at ambient temperature facilitate higher throughput, automated workflows that incorporate robotics. Our new formulation, MS β-glucuronidase, is optimized for ambient to 40°C and tailored to meet the high throughput needs of modern clinical and forensic testing labs. Optimization of the enzyme formulation provides an enzyme solution that serves as a reaction buffer, thus allowing testing labs to quickly perform the glucuronide-drug conversion at ambient temperature in less than 30 minutes for even the most difficult to hydrolyze drug glucuronides.
MS β-glucuronidase is a prebuffered, bench-stable, and automation-ready hydrolysis mix for urine drug testing application workflows. Our team of experts have designed MS β-glucuronidase so that it is suitable for a dilute-and-shoot workflow using the 10x urine dilution. Additionally, the recommended protocol reduces hands-on time and allows for a walk-away workflow, reducing cost per sample and increasing throughput. Discover MS β-glucuronidase and explore how it accommodates added steps like filtration or SPE if needed, without the need for oven incubation or centrifugation so that your specific needs are covered.
Manufacturer Merck | Available Worldwide
Be the first to review this product