Liver enzymes metabolize xenobiotics and endogenous metabolites by glucuronidation, making downstream quantification difficult. Most urine analysis and drug-testing workflows remove the glucuronide using β-glucuronidase. Importantly, the optimized activation temperature for β-glucuronidase impacts the suitability of a specific formulation for any given testing lab. Historically, our most popular recombinant β-Glucuronidase reagents have an optimal temperature of 70°C, which is perfect for the traditional testing lab workflow.
However, enzymes for workflows at ambient temperature facilitate higher throughput, automated workflows that incorporate robotics. Our new formulation, MS β-glucuronidase, is optimized for ambient to 40°C and tailored to meet the high throughput needs of modern clinical and forensic testing labs. Optimization of the enzyme formulation provides an enzyme solution that serves as a reaction buffer, thus allowing testing labs to quickly perform the glucuronide-drug conversion at ambient temperature in less than 30 minutes for even the most difficult to hydrolyze drug glucuronides.
MS β-glucuronidase is a prebuffered, bench-stable, and automation-ready hydrolysis mix for urine drug testing application workflows. Our team of experts have designed MS β-glucuronidase so that it is suitable for a dilute-and-shoot workflow using the 10x urine dilution. Additionally, the recommended protocol reduces hands-on time and allows for a walk-away workflow, reducing cost per sample and increasing throughput. Discover MS β-glucuronidase and explore how it accommodates added steps like filtration or SPE if needed, without the need for oven incubation or centrifugation so that your specific needs are covered.