Monolith X for Immobilization-Free Binding Affinity Measurements

Monolith is an easy-to-learn, low footprint, fluidics-free instrument that makes the perfect versatile tool for any laboratory.

Find Kds of a wide variety of targets and ligands while working in solution and without using lots of sample.

With in-solution, low-sample-consumption affinity measurements, it's possible to measure otherwise challenging interactions. Monolith X enables you to measure affinities of:

• Membrane proteins like GPCRs and ABC transporters
• IDPs such as Tau
• Proteins in lysate, serum, or media
• Ternary complex interactions
• Low-solubility proteins that require high detergent concentration
• Mass-change-independent interactions, such as small molecules or ions vs. large proteins

Enjoy approachable assay development with a fully guided process embedded in Monolith Control software. Monolith is an easy-to-learn, low footprint, fluidics-free instrument that makes the perfect versatile tool for any laboratory. Quantify low and high binding affinities, from low nM to mM, allowing you to detect strong and weak binders. Find Kds even when other methodologies fail, or validate affinities measured on another instrument. And, get more than just a Kd: study binding stoichiometry* and thermodynamic parameters*, assess relative affinities with competition assays, and characterize binding cooperativity*
*Requires offline data handling, not supported by Monolith software.

Monolith X is equipped with Spectral Shift Technology and MST/TRIC technology, the leading technologies for immobilization-free, low sample consumption alternatives to other affinity measurements.