Affinity measurements with the Monolith X
4 Jun 2024
Application Scientist Nathan Wallace, PhD, introduces the Monolith X instrument, emphasizing its features like easy assay development and quick KD measurement. It discusses the instrument's versatility in testing different molecules and buffers. The two biophysical modalities, spectral shift technology and MST, are explained. The process of sample preparation, software usage, and assay optimization is also covered.
About the company
NanoTemper Technologies
NanoTemper Technologies – We care about your research
We develop highly innovative instrument and software solutions for biomolecular analysis. Our global application centers ensure unparalleled customer care and support. Excellence. Focus. Passion. For your research. For Affinity. Stability. Conformation.
At NanoTemper Technologies, we are passionate about achieving excellence in everything we do. This has made us the partner of choice for thousands of researchers worldwide in the pharmaceutical and biotechnology industries as well as in academic settings. Our collaborative approach, science-driven insights, and high-quality, proprietary instruments and analytical technologies focus on helping researchers make the greatest impact – with maximum speed, efficiency and precision. A dedicated team of experts operates globally to offer you and your team individual application support and to ensure maximum instrument capability. Wherever you are, regardless of your focus...we care about your research.
NanoTemper Technologies is a market leader in providing high-quality instruments for biomedical research. The company’s products are used by thousands of scientists across four continents. With headquarters in Munich, Germany, and subsidiaries in the UK, Poland, US, Brazil and India, the company is rapidly expanding worldwide. Among others, NanoTemper Technologies has been awarded the German Innovation Award 2012, the Bavarian Export Prize 2013 and the Deutscher Gründerpreis (German Founders Award) 2014.
NanoTemper Technologies develops, produces and markets technologies and instruments for biomolecular analytics, from basic to preclinical research. The high-quality instruments are based on proprietary and innovative technologies:
Monolith: The Efficient Performer
MicroScale Thermophoresis (MST) technology is a unique method to study binding affinities in an easy, fast and precise way. Four Monolith Series instruments were introduced since 2010.
Seismos: The In-Depth Explorer
The recently acquired Surface Acoustic Wave (SAW) technology measures binding kinetics, and is sensitive to both mass and conformational changes
Prometheus: The Stability Expert
In 2015, a novel product line represented by the Prometheus NT.48 instrument was launched offering nanoDSF technology. This is the method of choice for measuring ultra-high-resolution protein stability in native conditions using intrinsic tryptophan and tyrosine fluorescence.
Visit NanoTemper Technologies to learn more about the MST, SAW and nanoDSF technologies. Schedule an instrument demonstration and explore the benefits of NanoTemper’s devices with your own samples.
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Monolith X for Immobilization-Free Binding Affinity Measurements
NanoTemper TechnologiesMonolith X is a capillary-based system that enables you to measure the strength of molecular interactions in solution, without the need for immobilization. Minimal impact fluorescent labelling empowers you to measure Kds between a wide variety of targets and ligands using low amounts of sample. Enjoy approachable assay development with a fully guided process embedded in the default software.
