Monoclonal Anti-α-Actinin (Sarcomeric) antibody produced in mouse by Merck KGaA, Darmstadt, Germany

Manufacturer Merck KGaA, Darmstadt, Germany  |   Model: A7811  |  Available Worldwide
clone EA-53, ascites fluid


Monoclonal Anti-α-Actinin (Sarcomeric) antibody produced in mouse by Merck KGaA, Darmstadt, Germany product image
Monoclonal Anti-α-Actinin (Sarcomeric) antibody produced in mouse

Merck KGaA, Darmstadt, Germany

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α-Actinin (ACTN2) is a 100kDa actin-binding protein that is found in muscle as well as non-muscle cells. In smooth muscles, α-actinin is present in dense bodies and plaques whereas in normal skeletal muscles, it is associated with z-discs that define muscle sarcomeres. ACTN2 is located on human chromosome lq42-q43. α-actinin has rod shaped N-terminal domain.

Mouse monoclonal anti-α-actinin (sarcomeric) antibody stains thymic myoid cells. The antibody exhibits wide reactivity with human and animal muscle tissue.

The antibody is specific for a-skeletal and a-cardiac muscle actinins. The antibody labels Z lines and dots in stress fibers of skeletal muscle in myotubes but does not react with non-skeletal muscle elements (e.g., connective tissue, epithelium, nerves, smooth muscle).

Mouse monoclonal anti-α-actinin (sarcomeric) antibody stains thymic myoid cells. The antibody exhibits wide reactivity with human and animal muscle tissue. α-actinin is detected predominantly in dense bodies and plaques which are characteristic of that tissue. Immunofluorescent labeling of a large variety of cells with anti a -actinin reveals an extensive association of the proteins with the actin containing stress fibers and, in particular, with their membrane-bound termini.

Monoclonal Anti-α-Actinin (Sarcomeric) (mouse IgG1 isotype) is derived from the EA-53 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified rabbit skeletal a-actinin.1 The isotype is determined using Mouse Monoclonal Antibody Isotyping Reagents, Catalog Number ISO2. Monoclonal Anti-α-Actinin (Sarcomeric) may be used for the localization of sarcomeric α-actinin using various immunochemical assays such as ELISA, dot blot, immunoblot, immunohistochemistry, and immunocytochemistry. The antibody is useful in the immunolocalization of α-actinin in normal and neoplastic cultured cells and tissues, and for studies on the state of sarcomeric muscle organization, in normal and pathological situations.

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Monoclonal Anti-α-Actinin (Sarcomeric) antibody produced in mouse by Merck KGaA, Darmstadt, Germany product image

Monoclonal Anti-α-Actinin (Sarcomeric) antibody produced in mouse

Manufacturer Merck KGaA, Darmstadt, Germany  |  Available Worldwide

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