xMAP® Technology uses color-coded microspheres to detect multiple protein or oligonucleotide analytes from a single aliquot of sample in a single reaction well, enabling you to generate more data while saving sample, time, and reagents. Depending on your targets and the application, there are five different microsphere types to select from to produce the best experimental results.
MagPlex-TAG™ are oligonucleotide-coupled magnetic polystyrene microspheres that have been dyed into spectrally distinct sets or “regions”, allowing them to be individually identified by an xMAP instrument. In the multiplex assay environment, the microspheres act as both the surface for the solution phase assay and the spectral identifier that the instrument detects. The open architecture of xMAP Technology enables users to build custom multiplex assays or select from a broad menu commercially available kits.
Each MagPlex-TAG microsphere has a unique 24 base DNA sequence, called an “anti-TAG,” covalently coupled to its surface. These beads enable the user to quickly and easily design custom bead arrays, simply by adding a complementary “TAG” sequence to the primer products or probes in their assay and hybridizing those TAGs to the anti-TAG sequence on the addressable microsphere. For a complete list of the TAG and anti-TAG sequences for each of the 150 available microspheres, visit the Luminex website.
MagPlex-TAG eliminates the need to couple oligonucleotides to the microspheres beads and are: