Klenow Fill-In Kit
Choose the Klenow Fill-In Kit to perform both partial fill-in reactions to prepare genomic DNA for insertion into the Lambda FIX II vector, and complete fill-in of a 5 overhang to generate blunt ends, to allow direct ligation. Or, use the kit to radioactively end label a 5 overhang with [alpha- 32 P]dNTP.
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Choose the Klenow Fill-In Kit to perform both partial fill-in reactions to prepare genomic DNA for insertion into the Lambda FIX II vector, and complete fill-in of a 5 overhang to generate blunt ends, to allow direct ligation. Or, use the kit to radioactively end label a 5 overhang with [alpha-32P]dNTP.
The Klenow Fill-in Kit contains Klenow fragment (125 U), dNTPs (separate 10 mM stocks), Klenow reaction buffer, predigested DNA, which can be used as a control, and sufficient material for 25 reactions.
Features:
- Perform partial fill-in reactions to prepare genomic DNA for insertion into the Lambda FIX II vector
- Perform a complete fill-in of a 5 overhang to generate blunt ends, to allow direct ligation
- Radioactively end label a 5 overhang with [alpha-32P]dNTP
- Includes predigested DNA that can be used as a control
For Research Use Only. Not for use in diagnostic procedures.
