Choose iScript Reverse Transcription Supermix for RT-qPCR for fast synthesis of first-strand cDNA for direct use in real-time qPCR. This single-tube supermix contains all the components for reverse transcription except RNA template.
Key Features and Benefits
Combined oligo(dT) and random primers provide unbiased representation of 5' and 3' regions
Contains RNase H+ MMLV reverse transcriptase and has a linear range of 1 µg–1 pg total input RNA
Amplify cDNA for genes with low expression levels
Fast 26-minute 1-tube cDNA synthesis protocol
50 reactions of no-RT controls for optional detection of gDNA amplicon contamination