iQue® Human T Cell Exhaustion Kit by Sartorius Group

Manufacturer Sartorius Group  |   Model: 97070  |  Available Worldwide
The Human T Cell Exhaustion Cell and Cytokine Profiling Kit is designed for ease of use in multiplexing markers of T cell exhaustion, phenotyping T helper and T cytotoxic cells, and bead-based measurement of secreted cytokines, all in the same assay. 


iQue® Human T Cell Exhaustion Kit by Sartorius Group product image
iQue® Human T Cell Exhaustion Kit
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This optimized assay offers these unique advantages:

  • Live immune cells are distinguished from dead or membrane compromised cells by staining with a fluorescent membrane integrity dye.
  • Cells are immunophenotyped by staining with a fluorescent antibody panel to distinguish CD3+ T cells, CD3- non-T cells, CD3+CD4+ helper T cells, and CD3+CD8+ cytotoxic T cells.
  • The panel also includes three markers of T cell exhaustion: PD-1, Tim-3, and Lag-3.
  • Optimized workflow enables the measurement of cytokines secreted by activated T cells (IFNγ and TNF) in the same assay well.
  • The capacity of the sample cells to proliferate can also be determined with an optional proliferation dye if desired (included with the kit).