Invitrogen™ Lipofectamine® 2000 by Thermo Fisher Scientific

Manufacturer Thermo Fisher Scientific  |   Model: 11668030  |  Available Worldwide
4.4
/
5.0
  |  2 reviews
The trusted, simple, broad-spectrum transfection reagent for most cell lines


Invitrogen™ Lipofectamine® 2000 by Thermo Fisher Scientific product image
Invitrogen™ Lipofectamine® 2000

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Average Rating: 4.4
2 Scientists have reviewed this product

5 out of 5
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2021

  • Organization: University of Szeged



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Great and robust transfection system.
Rating: 4.7

  • Application Area: Transfection

"We used Lipofectamine 2000 and 3000 and got the same results. They are pretty robust transfection agents in a variety of input nucleic acids. The protocol is easy to understand and use, you get the results within 4 hours."

Review date: 23 Jul 2021 | Invitrogen™ Lipofectamine® 2000
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University of Liverpool



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
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Lipofectamine 2000 achieves 95% transfection efficency.
Rating: 4.0

  • Application Area:Lentivirus production

"Online instructions were very clear to follow. Good transfection efficiency within 24hr period 95% in HEK293 cells GFP labelled calmodulin construct. Cells seeded at 9x10 6 cells within 10 cm dish. "

Review date: 22 Nov 2019 | Invitrogen™ Lipofectamine® 2000

Transfection commonly refers to the introduction of nucleic acids into eukaryotic cells, or more specifically, into animal cells. Invitrogen Lipofectamine® 2000 Transfection Reagent is a versatile transfection reagent that has been shown to effectively transfect a variety of payloads into the widest variety of adherent and suspension cell lines. Researchers use Lipofectamine® 2000 reagent for plasmid DNA transfection as well as siRNA- and shRNA-based gene knockdown experiments and gene expression studies. 

Lipofectamine® 2000 reagent delivers DNA or siRNA with excellent transfection performance for protein expression, gene silencing, and functional assays. At the foundation of the popularity of this broad-spectrum reagent is the reliably high efficiency and simple protocol for success across a broad range of cell lines. Lipofectamine® 2000 transfection reagent works effectively with all common cell lines as well as with many challenging ones and can be used in media with or without serum.

Offers:

  • Exceptional transfection efficiency in a broad range of cell lines with high levels of recombinant protein expression and a simple protocol

  • Superior performance for co-transfection of siRNA and plasmid DNA with reliable performance for high-throughput applications

  • Overwhelmingly more publications cite the use of Lipofectamine 2000 Transfection Reagent