Member since: 2019
Organization: University of Hyogo, Japan
Easy way to Western blot after the transfer of proteins onto membrane.
Application Area: Western blot
"Very easy, and rapid preparation for immunoblotting. You just set antibodies and wash buffer in the kit, then immunoblotting automatically proceeds."
Member since: 2015
Organization: University of Copenhagen
The best and coolest lab-instrument invention in years!
Application Area:Western Blot - We use it for semi-quantitative protein expression analysis
"iBind™ Flex Western Device is a fantastic, very easy-to-use device for quick Western Blot (WB) analyses. Though it is MUCH easier and quicker (2-2.5 hrs) to use than conventional WB methods, it is not as sensitive as our method when blotting against Strep. I only use our conventional method when I work with more-difficult-than-usual proteins. Apart from the aforementioned advantages, this device is brilliant when needing to analyse proteins/protein complexes with different tags, since you can run up to 6 different-tagged proteins at once. Also, no hazardous chemicals or reagents are used; cleaning and clean-up time is negligent. Just make sure that you a) rinse the blot really well with dH2O before development, and b) rinse the device really well with dH2O after use."
Member since: 2017
Organization: Instituto de Salud Carlos III
Very easy to use and effective for highly expressed proteins
Application Area:The system is especially useful for loading controls (e.g. actin) or highly expressed proteins.
"The system is very effective for highly expressed proteins (e.g. loading controls like actin). It may also be worthy to spend some time to set it up for those antibodies commonly used in your research. It s very easy to use and consumables are relatively cheap"
The iBind™ Flex Western System offers automated western blot processing and can accommodate various blot formats including midi, mini, and vertically cut strips. Based on Sequential Lateral Flow (SLF) technology, all blocking, antibody incubation and washing steps during immunodetection are hands-free. Compared to manual methods, the iBind Flex Western System requires less primary antibody, enables reproducible results and is compatible with downstream detection protocols. The iBind Western Starter Kit includes one iBind Flex Western Device , one box of iBind Flex Cards and one iBind Flex Solution Kit.