Invitrogen™ Dynabeads™ MyOne™ Streptavidin T1 by Thermo Fisher Scientific

Dynabeads MyOne Streptavidin T1 are the gold standard for isolation and handling of biotinylated nucleic acids, antibodies, or other biotinylated ligands and targets. The very high binding affinity of the streptavidin-biotin interaction (Kd=10^-15) is used in a vast number of applications. Benefits and features:

• Direct and fast isolation of any biotinylated molecule
• Flexible protocols with gentle and efficient liquid-phase reaction kinetics
• Low-charged and neutral beads, optimal for binding of proteins, peptides, and antibodies
• Their small but uniform size presents a high surface area per mg beads and a correspondingly high capacity for the target molecule
• Low sedimention rate, yet a high iron content, ensuring rapid magnetic separation
• Biomagnetic protocols are easily adapted to automated platforms
• High batch-to-batch reproducibility, securing consistent results in your application

About Dynabeads MyOne Streptavidin T1

These uniform and superparamagnetic beads are 1 µm in diameter, with a monolayer, not a multilayer, of recombinant streptavidin covalently coupled to the surface and further blocked with BSA. The monolayer of streptavidin leaves the vast majority of the biotin binding sites sterically available for binding, not only of free biotin, but also for binding of biotinylated ligands/targets. The beads show rapid liquid-phase reaction kinetics. Their specific and defined surface allow for efficient capture, separation, and downstream handling. The streptavidin monolayer ensures negligible leakage, and the lack of excess adsorbed streptavidin ensures batch consistency and reproducibility of your results. The 1 µm Dynabeads MyOne have a large surface area, high capacity, efficient magnetic pull, and a slow sedimentation rate during incubation. Tailor-made for use in automated protocols where high throughput is crucial.

Applications

Over the past 15 years, streptavidin-coupled Dynabeads have been used and cited for a very wide variety of applications. Key applications include preparing single-stranded DNA templates, isolation of RNA and DNA binding proteins, immobilization of large DNA fragments, purifying sequencing products, and the specific capture of nucleic acids. Easily adapted to automated processes. Dynabeads are used on more than 25,000 routine IVD instruments worldwide.

Binding capacity

The size of the molecule and the biotinylation procedure will affect the binding capacity. The capacity also depends on steric availability and charge interaction between bead and molecule and between molecules. There are two or three biotin binding sites available for each streptavidin molecule on the surface of the bead after immobilization. One mg of Dynabeads MyOne Streptavidin T1 typically binds:

• 950–1500 pmoles free biotin
• ~20 µg biotinylated IgG
• ~400 pmol biotinylated peptides
• ~20 µg ds-DNA
• ~400 pmol ss-oligonucleotides