Intercept® Blocking Buffers by LI-COR Biosciences

Manufacturer LI-COR Biosciences  |  Available Worldwide
4.8
/
5.0
  |  16 reviews
Try Intercept (TBS) Blocking Buffer or Intercept (PBS) Blocking Buffer for improved results in your quantitative Western blots and other immunoassays. Intercept Blocking Buffers provide excellent blocking performance with low background and low variability.  


Intercept® Blocking Buffers by LI-COR Biosciences product image
Intercept® Blocking Buffers
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Average Rating: 4.8
16 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2020

  • Organization: WSU



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Reliable results, no difference to LI-COR's former TBS buffer.
Rating: 5.0

  • Application Area: Analyze phospho proteins in brain samples

"Product is no worse in performance than the discontinued TBS blocking buffer (927-50000), if anything it gives sharper bands on the western blots with slightly brighter signal. Would recommend."

Review date: 19 May 2020 | Intercept® Blocking Buffers
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: Banck Center



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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These are excellent blocking buffers for western blotting.
Rating: 5.0

  • Application Area:Western blot

"We have used TBS Intercept and PBS-based Intercept blocking buffers for western blot analyses and in-cell westerns respectively. Both have worked great in our hands and are simple to use. Highly recommend."

Review date: 17 May 2020 | Intercept® Blocking Buffers
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: Scripps research



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great new product for blotting!
Rating: 5.0

  • Application Area:Western blotting detection

"Intercept is the new blocking buffer from LI-COR. It is a great reagent to minimize non-specific signal and obtain high quality data from western blots any time!"

Review date: 16 May 2020 | Intercept® Blocking Buffers
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: Gustavus Adolphus College



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Key to low background infrared western blots.
Rating: 4.7

  • Application Area:Analyze specific proteins by imumunodetection through western blot

"This product is available with different buffer chemistries that are commonly used in western blots, allowing the user to select a product that comes ready to use out of the bottle. It is particularly valuable as has been optimized for low background detection with infrared fluor-labeled antibodies. Customer service from Li-COR is second to none, whether by phone or email; I've often communicated directly with their application scientists. Of course, with any commercially prepared reagent, you pay more for the advantage of the time you save from having to optimize reagents you might prepare yourself. In this case, it is well worth the investment."

Review date: 15 May 2020 | Intercept® Blocking Buffers
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: Harvard



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Need this stuff.
Rating: 5.0

  • Application Area:Western blotting of cell and tissue samples

"This blocker is low background, high quality and easy to use. It provides consistency for a notoriously inconsistent technique."

Review date: 15 May 2020 | Intercept® Blocking Buffers
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: Amherst College



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great product - consistent high quality results; better value than previous line.
Rating: 5.0

  • Application Area:Western blot

"Great product! The Intercept blocking buffer is as high quality as the previous LI-COR blocking buffer it replaced, but it is slightly more affordable. We are happy with this product and plan to continue to use it."

Review date: 15 May 2020 | Intercept® Blocking Buffers
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: Omega Therapeutics



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great, easy to use blocking buffer!
Rating: 5.0

  • Application Area:Used as a blocking buffer in our in-cell western assays

"We used the LI-COR Intercept Blocking Buffers for our in-cell western work. It is easy to use (ready right out of the bottle!) and ships right when you order it. We got better results using this than we had with other blocking buffers (either homemade or from other manufacturers)."

Review date: 15 May 2020 | Intercept® Blocking Buffers
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: University of Florida



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Excellent blocking agent suitable for a wide variety of targets.
Rating: 5.0

  • Application Area:Western blotting - blocking agent

"We recently switched from the Odyssey blocking buffer (TBS) to the Intercept blocking buffer (TBS). We use this blocking buffer for all the blots we run in the lab including phosphorylated proteins. More importantly, we get nicer results with the Intercept than when using milk or BSA for the detection of phospho-proteins. We basically don't need to optimize the blocking conditions when using the Intercept. We normally get the 3 bottles since it is cheaper. I wish that they would give the option to buy the blocking buffer together with the membranes as they used to do with the Odyssey blocking buffer."

Review date: 15 May 2020 | Intercept® Blocking Buffers
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: Temple university



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Best results. Cannot imagine blots without them!
Rating: 5.0

  • Application Area:Western blots

"It is a super blocking buffer. It is fast, reliable with not too much background on the blot. It produces consistent results. I have tried reusing it once and I still got very good results. However, it cannot be reused multiple times. My blots are cleaner and nicer. It’s been 2 years since I've been using it and I’m quite impressed with the results. Will surely recommend to people looking for best."

Review date: 15 May 2020 | Intercept® Blocking Buffers
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: University of Pittsburgh



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Greater sensitivity with less background.
Rating: 5.0

  • Application Area:Virion protein expression

"Intercept blocking buffer works just as well, if not better, than it's predecessor, Odyssey Blocking Buffer. I have noticed higher sensitivity and less background with Intercept in my western blots."

Review date: 15 May 2020 | Intercept® Blocking Buffers


  • Status:

    Reviewer

  • Member since: 2020

  • Organization: Eidos Therapeutics



  • Ease of use
    3 out of 5
    After sales service
    3 out of 5
    Value for money
    3 out of 5
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Higher background.
Rating: 3.0

  • Application Area:Western blot PD assay

"Although somewhat similar to the Odyssey version, except Intercept has higher background in Western blots despite claim to be equivalent."

Review date: 15 May 2020 | Intercept® Blocking Buffers
  • Status:

    Advanced Reviewer

  • Member since: 2009

  • Organization: University of Minnesota



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Easy to use, effective blocking.
Rating: 5.0

  • Application Area:Western blot blocking buffer

"This product is very easy to use, lasts well in the refrigerator, and provides great control of background noise in PVDF fluorescent western blots. It is provided in a format that is easy to dilute and/or add additional detergent, etc. to and dilute antibodies in."

Review date: 15 May 2020 | Intercept® Blocking Buffers
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: GSK



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Totally recommend!
Rating: 5.0

  • Application Area:Immunoblots

"It truly is a great product! I get clear results after blocking with this buffer."

Review date: 15 May 2020 | Intercept® Blocking Buffers
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: umass



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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A bit pricey but works well, I wouldn't switch it.
Rating: 4.0

  • Application Area:Protein studies, western

"Nice product. Some antibodies still give background in this buffer, but in most cases the result is very nice."

Review date: 15 May 2020 | Intercept® Blocking Buffers
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: University of Maryland, Baltimore



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Reassured when we replicated results previously found using the Odyssey system.
Rating: 4.7

  • Application Area:Analyze protein by western blot

"Generally easy to use and so far has provided the same quality as the Odyssey system which I have previously used. Applied the same protocols to this buffer."

Review date: 15 May 2020 | Intercept® Blocking Buffers
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: NIH



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results!
Rating: 5.0

  • Application Area:Analyze proteins in cells

"It is great for protein detection in cells and tissues."

Review date: 15 May 2020 | Intercept® Blocking Buffers

Try Intercept (TBS) Blocking Buffer or Intercept (PBS) Blocking Buffer for improved results in your quantitative Western blots and other immunoassays. Intercept Blocking Buffers provide excellent blocking performance with low background and low variability.

Intercept Blocking Buffers are available in protein-based and protein-free formulations. TBS and PBS versions are available for both. So, optimize your Western blot blocking conditions for the best results and most reliable data. 
 

Product Overview

Intercept® Blocking Buffers by LI-COR Biosciences product image

Intercept® Blocking Buffers

Manufacturer LI-COR Biosciences  |  Available Worldwide

4.8 / 5.0 | 16 reviews