Intercept® Blocking Buffers by LI-COR Biosciences

"We have used TBS Intercept and PBS-based Intercept blocking buffers for western blot analyses and in-cell westerns respectively. Both have worked great in our hands and are simple to use. Highly recommend."

"Intercept is the new blocking buffer from LI-COR. It is a great reagent to minimize non-specific signal and obtain high quality data from western blots any time!"

"This product is available with different buffer chemistries that are commonly used in western blots, allowing the user to select a product that comes ready to use out of the bottle. It is particularly valuable as has been optimized for low background detection with infrared fluor-labeled antibodies. Customer service from Li-COR is second to none, whether by phone or email; I've often communicated directly with their application scientists. Of course, with any commercially prepared reagent, you pay more for the advantage of the time you save from having to optimize reagents you might prepare yourself. In this case, it is well worth the investment."

"This blocker is low background, high quality and easy to use. It provides consistency for a notoriously inconsistent technique."

"Great product! The Intercept blocking buffer is as high quality as the previous LI-COR blocking buffer it replaced, but it is slightly more affordable. We are happy with this product and plan to continue to use it."

"We used the LI-COR Intercept Blocking Buffers for our in-cell western work. It is easy to use (ready right out of the bottle!) and ships right when you order it. We got better results using this than we had with other blocking buffers (either homemade or from other manufacturers)."

"We recently switched from the Odyssey blocking buffer (TBS) to the Intercept blocking buffer (TBS). We use this blocking buffer for all the blots we run in the lab including phosphorylated proteins. More importantly, we get nicer results with the Intercept than when using milk or BSA for the detection of phospho-proteins. We basically don't need to optimize the blocking conditions when using the Intercept. We normally get the 3 bottles since it is cheaper. I wish that they would give the option to buy the blocking buffer together with the membranes as they used to do with the Odyssey blocking buffer."

"It is a super blocking buffer. It is fast, reliable with not too much background on the blot. It produces consistent results. I have tried reusing it once and I still got very good results. However, it cannot be reused multiple times. My blots are cleaner and nicer. It’s been 2 years since I've been using it and I’m quite impressed with the results. Will surely recommend to people looking for best."

"Intercept blocking buffer works just as well, if not better, than it's predecessor, Odyssey Blocking Buffer. I have noticed higher sensitivity and less background with Intercept in my western blots."