Status:
Advanced ReviewerMember since: 2016
Organization: University Of Miami
Best blocking buffer for Western blotting
Application Area: Western blotting
"Easy to use, reliable and consistently good quality. I have been using this product for 3 years and I have never have a bad experience."
Status:
ReviewerMember since: 2020
Organization: WSU
Reliable results, no difference to LI-COR's former TBS buffer.
Application Area:Analyze phospho proteins in brain samples
"Product is no worse in performance than the discontinued TBS blocking buffer (927-50000), if anything it gives sharper bands on the western blots with slightly brighter signal. Would recommend."
Status:
ReviewerMember since: 2020
Organization: Banck Center
These are excellent blocking buffers for western blotting.
Application Area:Western blot
"We have used TBS Intercept and PBS-based Intercept blocking buffers for western blot analyses and in-cell westerns respectively. Both have worked great in our hands and are simple to use. Highly recommend."
Status:
ReviewerMember since: 2020
Organization: Scripps research
Great new product for blotting!
Application Area:Western blotting detection
"Intercept is the new blocking buffer from LI-COR. It is a great reagent to minimize non-specific signal and obtain high quality data from western blots any time!"
Status:
ReviewerMember since: 2020
Organization: Gustavus Adolphus College
Key to low background infrared western blots.
Application Area:Analyze specific proteins by imumunodetection through western blot
"This product is available with different buffer chemistries that are commonly used in western blots, allowing the user to select a product that comes ready to use out of the bottle. It is particularly valuable as has been optimized for low background detection with infrared fluor-labeled antibodies. Customer service from Li-COR is second to none, whether by phone or email; I've often communicated directly with their application scientists. Of course, with any commercially prepared reagent, you pay more for the advantage of the time you save from having to optimize reagents you might prepare yourself. In this case, it is well worth the investment."
Status:
ReviewerMember since: 2020
Organization: Harvard
Need this stuff.
Application Area:Western blotting of cell and tissue samples
"This blocker is low background, high quality and easy to use. It provides consistency for a notoriously inconsistent technique."
Status:
ReviewerMember since: 2020
Organization: Amherst College
Great product - consistent high quality results; better value than previous line.
Application Area:Western blot
"Great product! The Intercept blocking buffer is as high quality as the previous LI-COR blocking buffer it replaced, but it is slightly more affordable. We are happy with this product and plan to continue to use it."
Status:
ReviewerMember since: 2020
Organization: Omega Therapeutics
Great, easy to use blocking buffer!
Application Area:Used as a blocking buffer in our in-cell western assays
"We used the LI-COR Intercept Blocking Buffers for our in-cell western work. It is easy to use (ready right out of the bottle!) and ships right when you order it. We got better results using this than we had with other blocking buffers (either homemade or from other manufacturers)."
Status:
ReviewerMember since: 2020
Organization: University of Florida
Excellent blocking agent suitable for a wide variety of targets.
Application Area:Western blotting - blocking agent
"We recently switched from the Odyssey blocking buffer (TBS) to the Intercept blocking buffer (TBS). We use this blocking buffer for all the blots we run in the lab including phosphorylated proteins. More importantly, we get nicer results with the Intercept than when using milk or BSA for the detection of phospho-proteins. We basically don't need to optimize the blocking conditions when using the Intercept. We normally get the 3 bottles since it is cheaper. I wish that they would give the option to buy the blocking buffer together with the membranes as they used to do with the Odyssey blocking buffer."
Status:
ReviewerMember since: 2020
Organization: Temple university
Best results. Cannot imagine blots without them!
Application Area:Western blots
"It is a super blocking buffer. It is fast, reliable with not too much background on the blot. It produces consistent results. I have tried reusing it once and I still got very good results. However, it cannot be reused multiple times. My blots are cleaner and nicer. It’s been 2 years since I've been using it and I’m quite impressed with the results. Will surely recommend to people looking for best."
Status:
ReviewerMember since: 2020
Organization: University of Pittsburgh
Greater sensitivity with less background.
Application Area:Virion protein expression
"Intercept blocking buffer works just as well, if not better, than it's predecessor, Odyssey Blocking Buffer. I have noticed higher sensitivity and less background with Intercept in my western blots."
Status:
ReviewerMember since: 2020
Organization: Eidos Therapeutics
Higher background.
Application Area:Western blot PD assay
"Although somewhat similar to the Odyssey version, except Intercept has higher background in Western blots despite claim to be equivalent."
Status:
Advanced ReviewerMember since: 2009
Organization: University of Minnesota
Easy to use, effective blocking.
Application Area:Western blot blocking buffer
"This product is very easy to use, lasts well in the refrigerator, and provides great control of background noise in PVDF fluorescent western blots. It is provided in a format that is easy to dilute and/or add additional detergent, etc. to and dilute antibodies in."
Status:
ReviewerMember since: 2020
Organization: umass
A bit pricey but works well, I wouldn't switch it.
Application Area:Protein studies, western
"Nice product. Some antibodies still give background in this buffer, but in most cases the result is very nice."
Status:
ReviewerMember since: 2020
Organization: University of Maryland, Baltimore
Reassured when we replicated results previously found using the Odyssey system.
Application Area:Analyze protein by western blot
"Generally easy to use and so far has provided the same quality as the Odyssey system which I have previously used. Applied the same protocols to this buffer."
Try Intercept (TBS) Blocking Buffer or Intercept (PBS) Blocking Buffer for improved results in your quantitative Western blots and other immunoassays. Intercept Blocking Buffers provide excellent blocking performance with low background and low variability.
Intercept Blocking Buffers are available in protein-based and protein-free formulations. TBS and PBS versions are available for both. So, optimize your Western blot blocking conditions for the best results and most reliable data.
Manufacturer LI-COR Biosciences | Available Worldwide
4.7 / 5.0 | 17 reviews