Member since: 2020
Organization: Mount Sinai Hospital
Great product, I highly recommend for western blotting and antibody preparation.
Application Area:Western blot analysis
"Great, cost-effective option for use as a blocking buffer in antibody preparation and western blotting protocols. Compared to other products, this blocking buffer reduces visual artifacts during imaging better."
Intercept T20 Antibody Diluents for use with Odyssey® Imaging Systems, are optimized for use with IRDye® Infrared Dye Reagents and other near-infrared fluorophores.
All Intercept T20 Antibody Diluents provide excellent performance for quantitative and chemiluminescent Western blots, as well as other immunoassays.
Antibody diluents are used for the dilution of primary antibodies and secondary antibodies to the working concentration required for your experiments. Antibody diluents improve the specificity of primary and secondary antibodies, reducing off-target effects. This reduces background due to non-specific binding.
Intercept T20 Antibody Diluents are preformulated in-house with Tween® 20, a non-ionic detergent. There’s no need to mix the diluent yourself, which saves you time and reduces potential variation.