Incucyte® Caspase-3/7 Red Apoptosis Assay Reagent by Sartorius Group

Manufacturer Sartorius Group  |   Model: 4704
One vial (20 µl) of 5 mM mix and read reagent in DMSO sufficient for 100-200 live-cell apoptosis tests in 96-well format.


Incucyte® Caspase-3/7 Red Apoptosis Assay Reagent by Sartorius Group product image
Caspase-3/7 Red Apoptosis Assay Reagent
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The Incucyte® Caspase-3/7 Red apoptosis assay reagent couples the activated caspase-3/7 recognition motif (DEVD) to NucView™633, a DNA intercalating dye to enable quantification of apoptosis over time. 

The Caspase-3/7 Red assay reagent is specially formulated for use in the Incucyte® live-cell analysis system and can be added directly to tissue culture wells using a no wash, mix and read protocol to acquire live cell images of cells undergoing caspase-3/7 mediated apoptosis. 

In addition, the Caspase-3/7 Red assay reagent is non-perturbing to cell growth and morphology. When added to tissue culture medium, this inert, non- fluorescent substrate crosses the cell membrane where it is cleaved by activated caspase-3/7 resulting in the release of the DNA dye and red fluorescent staining of nuclear DNA. 

Kinetic activation of caspase-3/7 can be monitored morphologically using live cell imaging, and quantified using the Incucyte® basic analyzer.