Status:
ReviewerMember since: 2020
Organization: The Institute Of Cancer Research
Great product for seamless cloning.
Application Area: Cloning
"Amazing for cloning, never fails, easy to use and very high success rate."
The In-Fusion HD EcoDry Cloning Plus kit enables high-efficiency, high-fidelity, directional cloning of one or more PCR fragments into any vector. The EcoDry Kit provides In-Fusion enzyme in a convenient, lyophilized (dry) format. In addition to the cloning kit, this package includes: a NucleoSpin Gel and PCR Clean-Up Kit, which can be used for both gel extraction and spin-column purification of PCR products; Stellar Competent Cells, an E. coli HST08 strain that provides high transformation efficiency paired with blue-white screening capability when used with pUC plasmid vectors; and CloneAmp HiFi PCR Premix, a convenient high-fidelity PCR 2X master mix that provides exceptionally accurate and efficient DNA amplification.
In-Fusion HD EcoDry Cloning Plus systems include all the reagents you need—including the cloning enzyme mix, PCR enzyme, competent cells, and gel extraction kit.