Human TNFRSF10B knockout HeLa cell line
Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1
Sanger Seq - TNFRSF10B knockout HeLa
WB - TNFRSF10B knockout HeLa
The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our Product Directory.
Cell line: HeLa
Number of cells: 1x106 cells/vial, 1 mL
Cell type: Epithelial
Tissue type: Cervix
Disease: Adenocarcinoma
Organism: Human
Gender: Female
Antibiotic resistance: Puromycin 1 µg/ml
Adherent: Yes
STR analysis: Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8, 12 CSF1PO: 9, 10
Passage number: <20
Biosafety level: 2
Viability: ~90%
Mycoplasma free: Yes
Storage: Shipped on dry ice. Store in liquid nitrogen.
Buffer constituents: 8.7% DMSO, 2% cellulose, methyl ether
Knock-out cell lines: A comprehensive guide to use and benefits
Gene knock-out (KO) technology and the generation of KO cell lines has been an essential tool in biology for studying genes of unknown function. However, the advent of CRISPR-Cas9 KO technology is expanding the range of cell lines available for research far beyond previous offerings, rendering them cheaper and more reliable through advancements such as direct injection.
This eBook provides an informative guide on how ready-made KO cell lines benefit workflows, provide quality control, and deliver confidence in results. Download now for insights including:
- The importance of robust KO validation
- Sourcing the right KO cell lines for your program
- gRNAs for increased gene-editing success
- Investigating signaling pathways such as PD-L1 and TRAIL
Knock-out cell lines and their use in signaling pathways
Knock-out (KO) cell lines are powerful tools to study disease mechanisms and the effect of gene disruption in signaling pathways. Using a KO cell line of a TRAIL death receptor, this application note demonstrates how you can use KO cell lines to investigate the relationship between gene and phenotype.
Have you read the new eBook on knock-out cell lines?
Explore a compendium full of tips, tricks, and tools, and explore cutting-edge research on KO cell lines
