Status:
ReviewerMember since: 2018
Organization: Public Welfare Organization
Very effective instrument
Application Area: Europe and America
"This product is good and easy to use. It also give high quality results"
Status:
ReviewerMember since: 2019
Organization: Azire Integrated Healt Center
Perfect product, I recommend this for other scientists.
Application Area:Daily use
"An important tool for studying enzyme kinetics and screening small molecular inhibitors used for discovering drugs. The Green Cathepsin B Assay permits scientists to discover and keep under surveillance intracellular cathepsin activity over some period in vitro with the use of whole live cells. This instrument is easy and straightforward, one can adapt it to a 96-well plate assays."
Status:
ReviewerMember since: 2019
Organization: St.marys College Of Sciences
This product is specially designed for the experimental studies.
Application Area:To enquiry the activities to of cathespian inside the cell
"Yes I recommend it to others because it is easy to use .. And it can also use to check the activity and different reactions carried out during the whole process."
Status:
ReviewerMember since: 2019
Organization: Peshawar University
Good quality product. Like it.
Application Area:Used once a day
"Very effective for immune system. High quality product. Easy to use and useful for anyone."
Cathepsins are a group of protease enzymes typically found in the lysosome. Cathepsin B plays important roles in many cellular functions (like intracellular protein degradation, antigen processing, etc.). Elevated cathepsin activity is associated with many disease states such as cancer, Alzheimer’s disease, and so on.
The Green Cathepsin B Assay contains Rhodamine 110 Cathepsin B Substrate, which is a non-cytotoxic and cell membrane permeant substrate that fluoresces green upon cleavage by active cathepsins. To use Rhodamine 110-(RR)2, add the substrate directly to the cell culture medium, incubate, and analyze. Because the substrate is cell permeant, it easily penetrates the cell membrane and the membranes of the internal cellular organelles – no lysis or permeabilization steps are required. If active cathepsin enzymes are present, the quenched Rhodamine 110-(RR)2 substrate is cleaved, resulting in an increase in green fluorescence signal. Samples can be analyzed by flow cytometry or fluorescence microscopy. Hoechst 33342 is included in the kit to label nuclei.