Member since: 2006
Organization: Universidade Federal do Acre
Application Area: Cancer Research
"GoTaq® 2-Step RT-qPCR System is a kind of product I recommend to any PCR routine laboratory; It made my work easy and I had good results using it. "
Member since: 2007
Organization: King Abdullah International Medical Research Center
Application Area:Real Time PCR
"This kit is super easy to use and setup from RNA to qRT-PCR. My only concern was the need of standard RNA which I didn’t have. If they can include it in the kit, it will be fantastic."
The first step consists of cDNA synthesis using the GoScript™ Reverse Transcription System. Optimized reagents and ultra-active, GoScript™ Reverse Transcriptase result in high-efficiency, full-length cDNA synthesis from rare and abundant targets with up to 5 µg of RNA input. In the second step, cDNA is quantified using the GoTaq®qPCR Master Mix.
Formulated with a hot-start DNA polymerase, the GoTaq® qPCR Master Mix is optimized for reproducibly specific, sensitive, highly efficient qPCR using the standard or fast mode of a real-time PCR instrument. The GoTaq® qPCR Master Mix contains BRYT™ GREEN dye which can be twice as bright as of SYBR® Green I. The superior performance of these two steps allows researchers to observe earlier quantification cycle values over broader linear ranges.
The GoTaq® 2-Step RT-qPCR System provides a ready-to-use kit for analyzing a wide range of RNA targets by combining the high-activity of GoScript™ Reverse Transcription System with the ultra-bright fluorescence of GoTaq® qPCR Master Mix. The system is robust, requires little optimization, is flexible for a variety of applications, and carries the PCR performance guarantee.