Gator™ AAVX Probes from Gator Bio by Gator Bio

Manufacturer Gator Bio  |  Available Worldwide
 Gator Bio BLI Probes for Quantitation of AAV Serotypes


Gator™ AAVX Probes from Gator Bio by Gator Bio product image
PL168-160017: GatorTM AAVX Probe
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Introduction

Adeno associated virus (AAV) capsids are a leading modality for in vivo gene delivery. Complete and precise characterization of capsid particles, including capsid and vector genome concentration, is necessary to safely and efficaciously dose patients. In virus development and production, it is important to determine the virus concentration at different stages of the process, to optimize the clone used as well as the production yields. Biolayer Interferometry (BLI) using AAV specific biosensors is a rapid label-free method for quantitation of AAV serotypes.

GatorTM AAVX Probes

The Gator AAVX probes are a high specificity antibody-based biosensors that enable direct capture and quantitation of different serotypes of AAV in crude lysates, column eluates, cell lysates and cell culture supernatants, serving as an alternative to traditional time-consuming analytical methods, such as qPCR, ddPCR, Dot blot and ELISA. The Gator AAVX probe uses proven CaptureSelect™ (Thermo Fisher Scientific) high affinity and high specificity anti-AAVX antibody.

Performance Summary

  • Dynamic range: 1 x 109 –1 x 1013 vp/mL for most AAV serotypes
  • Throughput: 8 samples in10 minutes, 96 samples in 120 minutes
  • Limit of detection: typically, 5 x 108 vp/mL (serotype dependent)
  • Crude sample tolerant
  • Stable over broad pH range
  • Cost effective - Reusable at least 10 times by regeneration